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Protocols

  • SOP for regular proteomics sample preparation: cell lysis, protein digestion, and peptide desalting

  • Protocol for on-filter in-gel (OFIG) protein digestion

  • Protocol for on-filter in-cell (OFIC) digestion of C. elegans worms

  • Protocol for on-filter in-cell (OFIC) digestion of intact cells

  • Protocol for on-filter in-cell (OFIC) digestion of plant leaf

  • Protocol for on-filter in-cell (OFIC) digestion of plant pollen

  • More proteomics methods (UWPR)

  • Recommended consumables and supplies for proteomics experiments

  • To minimize plastic contamination, please use the following items for your sample preparation:
    – Low binding or maximum recovery tubes and pipette tips.
    – Glass containers for buffer storage.
    – Please see the recommended consumable and supplies above.

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