Equipment
Equipment List (Lu Lab: Room 221 & 224. Wang Lab: Room 202, 203 & 327):
| Category | Item description | Vendor | Specs | Room |
|---|---|---|---|---|
| MicroCT | Scanner uCT35 | Scanco | Three X-ray energy level; voxel resolution up to 3 micron; field of view up to 70 mm; various sized of holders; controller for imaging/reconstruction/evaluation software; remote access; 10TB storage; tape driver for backup. | SPL203 |
| Post-processing suite | custom | Three networked high-speed PC (Intel Core i7) installed with Simpleware ScanIP (converting microCT to FEM meshes), Hypermesh (for meshing and elastic FEA package), and freeware FEBio (for biphasic and nonlinear FEA) | SPL203 | |
| Microscope, Fluorescent Microscope, Confocal Microscope, and Accessories | Epifluorescence | Zeiss | Upright; White field; fluorescence (DAPI, FITC, Cy3 filter sets); Color Axio CamMRc 5. | SPL202 |
| Epifluorescence | Zeiss | Inverted; White field; fluorescence (DAPI, FITC, Cy3 filter sets); Monochrome Axio CamNRc; Motorized stage | SPL202 | |
| Epifluorescence | Zeiss | Imager A2; Upright; White field; fluorescence(DAPI, FITC, Cy3 filter sets); Colored Axio CamMRc 5 | SPL327 | |
| LSM 510 confocal (for FRAP, intracellular calcium and regular imaging) | Zeiss | Observer.Z1 (inverted configuration) with a lens Inverter to convert to upright; supported on Kinetic System air table; three PMT detectors for reflection/fluorescence and transmission lights; laser lines of Argon-458, 477, 488, 514nm; DPSS 516nm; HeNe 633nm; Diode 405-30nm | SPL202 | |
| Stereomicroscope | Olympus | SZx7; A wide 7:1 zoom; additional light source | SPL327 | |
| Fluorescent microscope DMI6000B | Leica | Inverted; Differential Interference Contrast; Ratiometric Fluorescence; Fluorescence (Texas Red, FITC, DAPI); High Speed EMCCD Camera; 3-Way Motorized Stage; Environmental Chamber for Long-Term Live Imaging | SPL221 | |
| Fluorescent light source | Lambda | DG-4 Ultra high speed wavelength switcher | SPL221 | |
| Live Imaging System on Microscope | Isofluorane anesthesia machine | System Specialties | For live animal studies, rodents are induced and maintained in anesthesia under mixture of isofluorane and oxygen | SPL202 |
| Environmental Chamber (for calcium signaling) | Haison | Temperature and CO2 are controlled by pumping hot air and infusion of 5%CO2 and 95% air inside a plastic enclosure around the LSM510 confocal. | SPL202 | |
| PECON Incubation System 2000 | PeCon GmbH | For cell and culture on the microscope; highly precise temperature sensor; LCD display and foil keypad; standalone or connected to PC; on stage heating plate; | SPL202 |
| Category | Item description | Vendor | Specs | Room |
|---|---|---|---|---|
| Mechanical Testing Devices | Bose Electroforce TestBench LSM1 (for in vivo loading) | Bose | Electromagnetic motor (peak force 225N, 0.5% resolution, 200Hz) with embedded LVDT sensor (2.5mm stroke, 0.5% resolution); both load and displacement control; reaction brackets with load cells (5, 10, 50lb); customized immersion chambers with compression and tension fixtures; WinTest controller and software; customized xyz translational platform for positioning | SPL202 |
| Bose Electroforce TestBench LSM0 (for in situ imaging) | Bose | Electromagnetic motor (peak force 22N, 0.5% resolution, 20Hz) with embedded LVDT sensor (0.5mm stroke, 0.5% resolution); both load and displacement control; reaction brackets with load cells (5lb); customized immersion chambers with compression and tension fixtures; WinTest controller and software; customized xyz translational platform for positioning beside confocal. | SPL202 | |
| TA RSA-G2 Mechanical Dynamic Analyzer (for mechanical testing) | TA Instruments | Table top; dual motor/transducer design; open testing gap; step motor for head adjustment; 35N max load/10pN resolution; 1.5mm displacement/1nm resolution; displacement/load control modes; up to 100 Hz; oven temp up to 300oC; grips for 3-point bending and tension/compression in solutions; online data report in TRO software | SPL202 | |
| Piezo-positioning /loading system | Custom | For simultaneous imaging on either upright or inverted microscope stages. Include PIHera nanopositioning stage (p-625) with 0.5mm stroke and internal capacitive sensor and PI amplifier/servo-controller; Custom LabView based control software and water baths for imaging and loading fixtures. | SPL202 | |
| Instron 5848 MicroTester | Instron | Two configurations (vertical and horizontal) 1-100 lbs load cells (0.1% accuracy) | SPL221 | |
| Instron ElectroPuls | Instron | ElectroPuls E3000 with environmental chamber | SPL224 | |
| microTesters* | Custom | Two micro-loading devices for compression test and mechanical stimulation of cartilage explants. Located in biological safety hoods. | SPL221 | |
| Triobometer | Custom | Tribometer for friction test. Measure the friction coefficient of cartilage surface in various loading/sliding profiles | SPL224 | |
| Microindenter* | Custom | Micro-loading device for indentation; 10 gram force load cell; 60 nm displacement resolution; Compact size fits in biological safety hood and incubator | SPL224 | |
| Optical Strain Mapping System | VIC-3D microscope system | Correlated Solutions | For mapping 3D surface strain on curved samples; includes a dual high-resolution camera mounted on a stereomicroscope; field of view (0.2mm-7mm); resolution 100 microstrain; PC-based control system for camera calibration; image correlation software for 3D strain mapping; accessories include airbrush for pattern generation, XYZ table, calibration grids. | SPL202 |
| Physiological Manipulation | PHD Ultra Syringe Pump | Harvard Apparatus | For perfusion of tissues and cells; microfluidic applications; drug deliver; Infuse/withdraw programmable; from pico-liter to 220ml/min flow rates; advanced programming without PC; Color LCD touch screen; vertical and horizontal orientation | SPL202 |
| Category | Item description | Vendor | Specs | Room |
|---|---|---|---|---|
| Sample Preparation, Histology, & Histomorphometry | IPC High Precision MultiChannel Dispenser | ISMATEC | For perfusion of tissue samples and cells; peristaltic pump with low pulse; Flow rate from 0.002-44ml/min; remote controllable. | SPL202 |
| Mikro-Tip catheter blood pressure measuring system | Millar | Electrophysiology catheter (SPR-1000) with high precision and high frequency (>300 Hz) measurement; signaling conditioner, calibration, data display and acquisition included in PCU-2000 box | SPL202 | |
| Manipulators | Narishige | M-152 manual manipulator attached to microscope stage or standalone pole; MMO-220 single axis oil hydraulic micromanipulator for fine control. | SPL202 | |
| Diamond saws | Buehler | Three Isomet (low speed and 1000 precision) saws to cut large and small samples; multiple fixtures with various degrees of freedom; water baths; 3”-7” diamond blades | SPL224, SPL327 | |
| 3D Milling machine | Roland | Table top 3D milling machine for creating planar and 3D samples | SPL327 | |
| Plastic embedding system | UD | An ultrasound heating bath for PMMA solutions; A Fisher Isotemp oven for curing plastics; a custom made polishing stations for polishing thin (50-200 micron) sections; graded sand papers and polishing media. | SPL327 | |
| Histology staining station | Fisher | Racks of staining dishes containing various agents; chemical hood | SPL327 | |
| OsteoMeasure XP bone histo-morphometry system | OsteoMetrics | Extensive interactive bone histomorphometry analysis system with high resolution digital color video camera (4080×3072). | SPL327 | |
| Biological Safety Cabinets | NuAire | Two Type A2 Class II Biological Safety Cabinets; Six Feet | SPL221 | |
| Tissue Culture | CO2 Incubators | Thermo Scientific | Two Heracell Incubators; Pure Copper Interior; Rapid Humidity Recovery; CO2 and O2 control | SPL221 |
| Biological Safety Cabinet I | NUAIRE | CellGard NU-480-600 Class II, 6 feet, Type A2 | SPL221 | |
| Biological Safety Cabinet II | SterilGARD | SterilGARD, Class II, 4 feet, Type A/B3 | SPL221 | |
| Water Bath | Thermo Scientific | Two Isotemp water bath | SPL221 | |
| Centrifuge | Thermo Scientific | Sorvall Legend Centrifuge; Ventilated; 15.2k rpm; Digital Control | SPL221 | |
| Oven | Lab companion | Forced convection oven, 3.6 cu.ft | SPL221 | |
| Autoclave | VWR | Accu sterilizer AS10 | SPL221 | |
| Microscope | Leica | DM IL LED, phase contrast | SPL221 | |
| Bioreactor | Custom | Bioreactor for Cartilage-Bone Explant Culture; Circulating Medium Supply System | SPL221 | |
| Zen 2010 software/FCS | Zeiss | Full license for confocal image analysis including FCS and STICS packages | SPL206 | |
| Software, Server, Workstation | Volocity | PerkinElmer | 3D reconstruction and rendering based on 2D series of images; Quantitation offers object identification, measurements, tracking. | SPL206 |
| Various workstations for imaging analysis, FEM simulation, CAD, and statistical analysis | HP | Two Workstations with Dual Intel Xeon E5606 2.13 8MB/1066 CPU; 48GB DDR-3 RAM; 160GB+1000GB HDD | SPL206, SPL224 | |
| Dell | Precision T7500 with a Wacom tablet for analyzing microCT 3D data and tracing ROI on 2D images | SPL206 | ||
| Dell | Two Precision T3500 and two T3400 PCs for numerical simulations running MATLAB, Fortran, and C | SPL206 | ||
| Custom Built | Eight custom built multi-core workstations for finite element simulation with FEBio installed | SPL224 | ||
| Synology | DS415+ NAS system for data storage and backup | SPL224 | ||
| Freezer and Refrigerator | Subzero Freezer | Thermo Scientific | Two Revco Elite PLUS, -86 degree, 25 cu ft | SPL221, SPL327 |
| Subzero Freezer | Panasonic | MDFU53VAPA – VIP ULTRALOW FREEZER UPRIGHT 18CUFT 115V | SPL202 | |
| Refrigerator/freezer combo | Panasonic | MPR715FPA – REFRIGERATOR 14.7CUFT FREEZER 6.2CUFT CO | SPL203 |
| Category | Item description | Vendor | Specs | Room |
|---|---|---|---|---|
| Chemical Assay | Fluorescence Microplate Reader | Molecular Devices | Gemini EM | SPL224 |
| Absorbance Microplate Reader | Molecular Devices | SpectraMax 340PC | SPL224 | |
| Homogenizer | Scientific | BeadBug Microtube D1030 for tissue homogenizer | SPL224 | |
| Thermomixer | Eppendorf | Thermomixer R (1.5ml) for PCR | SPL224 | |
| Incubating shaker | Thermo Scientific | MaxQ4450 Benchtop incubating shaker, digital | SPL224 | |
| Rocker (2) | Scilogex | SK-O180-Pro Orbital Digital Shaker | SPL224 | |
| Balance I | Mettler Toledo | XS64 | SPL224 | |
| Balance II | Denver | TP-3102 | SPL224 | |
| Ultrasonic Cleaner | Kendal | 6 Liters 380 Watts Heated ultrasonic cleaner | SPL224 | |
| Stir Hot Plate | Fisher | Four 7×7 hot plates | SPL224 | |
| Roller Pump | ISMATEC | Low-Flow 24 Channel roller pump with computer control | SPL224 | |
| PCR and Western Blot | Real Time PCR system | ABI | QuantStudio 3 Real-Time PCR System | SPL224 |
| Fluorescent Imaging System | BIORAD | Gel Doc System with CCD, high-resolution, high-sensitivity detection technology, support multiple detection methods | SPL224 | |
| Electrophoresis and blotting system | BIORAD | 10-well, 1.0 mm, 4-gel system (#165-8001) and blotting module (#170-3935) | SPL224 | |
| Power Supply | BIORAD | Universal Power Supply #164507 | SPL224 | |
| Miscellaneous | Glassware Washer | Labconco | SteamScrubber DI water glassware washer | SPL221 |
* If you are interested in building a similar loading device, we can share with you the design, part numbers and Labview control program.
** If you need any user feedback info about the devices, feel free to contact Dr. Lu (xlu@udel.edu)