Microbes control most biogeochemical activity either by directly by metabolic transformations of chemicals or indirectly by altering pH, ion concentration, and diffusion which therefore affect chemical speciation and transport in the environment. So, knowing about the microbiome at a site and its functional potential for metabolic transformation is a key part of wholistic biogeochemical models. This core seeks to provide data on microbiome structure and functional potential for important elements: C, N, S, P, and As. We have also assembled a set of primers to test for the presence and diversity of toxigenic cyanobacteria. More information on the primers and protocols we use are below. Don’t know where to start? Email us for a consultation at microbiome@udel.edu
Sample Preservation Protocol:
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Standard Panel Primers: These amplify genes enccoding key enzymes in pathways of interest selected by a survey of Project WiCCED researchers.
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Cyanobacterial Panel Primers: These amplify genes for targeted analysis of cyanobacterial populations and toxins. We’re using these to survey many water samples collected for the Delaware Microbiome Project.
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