Vitae

Professional Experience:
September 2011 – present
Professor of Chemistry & Biochemistry,  University of Delaware

September 2008 – August 2011
Director of Graduate Studies and Assistant Chair of the Department of Chemistry & Biochemistry,  University of Delaware

September 2004 – August 2011
Associate Professor of Chemistry & Biochemistry,  University of Delaware

September 1998 – August 2004
Assistant Professor of Chemistry & Biochemistry,  University of Delaware

July 1994 – July 1998
Brandeis University, Structural Biology Laboratory, Postdoctoral Fellow
Sponsors: Gregory A. Petsko and Dagmar Ringe
Area of study: x-ray crystallography

January 1994 – June 1994
Williams College, Department of Chemistry,
Visiting Assistant Professor
Teaching experience: a senior honors seminar course in biochemistry & molecular biology and general chemistry labs

September 1990 – December 1993
University of California, Berkeley, Department of Chemistry
National Institute of General Medical Sciences, Postdoctoral Fellow,
Sponsor: Judith P. Klinman.
Area of study: enzyme kinetics and molecular biology

Education:
September 1986 – August 1990
Brown University, Department of Chemistry, Ph.D.
Advisor: Vernon E. Anderson.
Thesis title: “The Chemical Mechanism of Crotonase”
Teaching experience: teaching assistant for general and organic chemistry

September, 1982 – May, 1986
University of Massachusetts, Amherst
B.S. Chemistry, B.S. Biochemistry, Cum Laude

Invited Talks:
March 1996, Univ. of Rochester Medical School, Dept. of Biophysics, Rochester, NY
March 1996, Center for Advanced Research in Biotechnology, Rockville, MD
January 1997, Univ. of Utah Medical School, Dept. of Biochemistry, Salt Lake City, UT
February 1997, Medical College of Wisconsin, Dept. of Biochemistry, Milwaukee, WI
February 1997, Univ. of North Carolina, Dept. of Pharmacy, Chapel Hill, NC
February 1997, Penn State Univ., Dept. of Biochem. and Mol. Biol., State College, PA
May 1997, University of Vermont, College of Medicine, Rutland, VT
August 1997, ACS Biological Division Meeting, San Francisco, CA
December 1997, Univ. of Delaware, Dept. of Chemistry and Biochemistry, Newark, DE
January 1998, Univ. of Miami, Dept. of Biochemistry, School of Medicine, Miami, FL
January 1998, Oregon State Univ., Dept. of Biochemistry & Biophysics, Corvallis, OR
March 1999, Dupont Stine-Haskell Research Center, Newark, DE
September 2000, Dupont Experimental Station, Wilmington, DE
February 2001, Symposium speaker for Biophysical Society Meeting, Boston, MA
March 2001, Villanova, Dept. of Chemistry, Philadelphia, PA
March 2001, Wake Forest Univ. School of Med., Dept. of Biochem., Winston Salem, SC
May 2001, Delaware Valley Enzymology Club, Wilmington, DE
July 2001, Case Western University, Department of Biochemistry, Cleveland, OH
April 2002, Rutgers University, Department of Chemistry, Newark, NJ
December 2002, Delaware Biotechnology Institute, Newark, DE
February 2003, North Carolina State University, Dept. of Biochemistry, Raleigh, NC
March 2003, University of Toledo, Dept. of Chemistry, Toledo, OH
April, 2003, University of Iowa, Dept. of Chemistry, Iowa City, IA
April 2003, University of Maryland, Dept. of Chem. and Biochem., College Park, MD
November 2003, Pittsburgh Diffraction Conf., Rutgers Univ., New Brunswick, NJ
April 2004, First Int. Conference on Paraoxonases, Univ. of Michigan, Ann Arbor, MI
July 2004, FASEB Conference on Phopholipases, Pine Mountain, GA
October 2004, 2nd Int. Conf. on PLA2, Berlin, Germany
November 2004, Colgate University, Dept. of Chemistry, Hamilton, New York
April 2005, University of Pennsylvania, Institute for Environmental Med., Philadelphia, PA
May 2006, speaker at the 89th Canadian Chemistry Conference in Halifax, Nova Scotia
November 2006, the Institut de Biologie Structurale of the CNRS, Grenoble France
May 2007, 3rd Int. Conf. on PLA2 and Lipid Mediators, Sorrento, Italy
August 2007, 2nd Northeast Regional IDeA Meeting, Burlington, VT
March 2008, GlaxoSmithKline, King of Prussia, PA
March 2009, Penn State, Hershey, PA
September 2009, Delaware Valley Enzymology Club, Media PA
October 2009, Georgetown University, Dept. of Chemistry, Georgetown, DC
November 2009, Drexel University College of Medicine, Dept. of Biochemistry and Molecular Biology, Philadelphia, PA
February 2010, Trinity University, Department of Chemistry, San Antonio, TX
February 2010, Univ. of Texas Health Science Center, San Antonio, Dept. of Biochemistry, San Antonio, TX
February 2010, The City College of CUNY, Department of Biochemistry, New York, NY
March 2010, Utah State Univ., Department of Chemistry and Biochemistry, Logan, UT
July 2010, GRC, Enzymes, Coenzymes & Metabolic Pathways, Waterville Valley, NH
October 2010, Delaware Valley Enzymology Club, Media, PA
February 2011, University of Massachusetts, Dept. of Chemistry, Amherst, MA
April 2011, Wake Forest School of Medicine, Winston-Salem, NC
April 2011, Klinman Symposium, U.C. Berkeley, CA
April 2012, Chemistry-Biology Interface Seminar, Univ. of Delaware, Newark, DE
July 2012, Fifth International Conference on Paraoxonases (5PON), Columbus OH

Publications:
B. J. Bahnson and V. E. Anderson* (1989) Isotope effects on the crotonase reaction, Biochemistry 28, 4173-4181.

V. E. Anderson*, B. J. Bahnson, I. D. Wlassics, and C. T. Walsh (1990) The reaction of acetyldithio-CoA, a readily enolized analog of acetyl-CoA with thiolase from Zoogloea ramigera, Journal of Biological Chemistry 265, 6255-6261.

B. J. Bahnson and V. E. Anderson* (1991) Crotonase-catalyzed beta-elimination is concerted: A double isotope effect study Biochemistry 30, 5894-5906.

B. J. Bahnson, D.-H. Park, K. Kim, B. V. Plapp, and J. P. Klinman* (1993) Unmasking of hydrogen tunneling in the horse liver alcohol dehydrogenase reaction by site-directed mutagenesis, Biochemistry 32, 5503-5507.

R. L. D’Ordine Jr., B. J. Bahnson, P. Tonge, and V. E. Anderson* (1994) Enoyl coenzyme-A hydratase catalyzed exchange of the alpha-protons of coenzyme-A thiol esters: A model for an enolized intermediate in the enzyme catalyzed elimination?, Biochemistry 33, 14733-14742.

B. J. Bahnson and J. P. Klinman* (1995) Hydrogen tunneling in enzyme catalysis, Methods in Enzymology (D. L. Purich ed.), 249, 373-397.

B. J. Bahnson, T. D. Colby, J. K. Chin, B. M. Goldstein, and J. P. Klinman* (1997) A link between protein structure and enzyme catalyzed hydrogen tunneling, PNAS 94, 12797-12802.

T. D. Colby, B. J. Bahnson, J. K. Chin, J. P. Klinman, and B. M. Goldstein* (1998) Active site modifications in a double mutant of liver alcohol dehydrogenase: structural studies of two enzyme-ligand complexes, Biochemistry 37, 9295-9304.

C. J. Jeffery, B. J. Bahnson, W. Chien, D. Ringe and G. A. Petsko* (2000) The crystal structure of rabbit phosphoglucose isomerase; a glycolytic enzyme that moonlights as neuroleukin, autocrine motility factor and differentiation mediator, Biochemistry, 39, 955-964.

Y. H. Pan,  T. M. Epstein, M. K. Jain, and B. J. Bahnson* (2001) Five coplanar anion binding sites on one face of phospholipase A2:  relationship to interface binding, Biochemistry, 40, 609-617.

T. M. Epstein, Y. H. Pan, S. P. Tutton, M. K. Jain, and B. J. Bahnson* (2001) The basis for k*cat impairment in prophospholipase A(2) from the anion-assisted dimer structure, Biochemistry, 40, 11411-22.

B. J. Bahnson*, V. E. Anderson, and G. A. Petsko (2002) Structural Mechanism of Enoyl-CoA Hydratase: Three Atoms from a Single Water are Added in Either an E1cb Stepwise or Concerted Fashion, Biochemistry 41, 2621-2629.

R. L. D’Ordine, J. Pawlak, B. J. Bahnson V. E. Anderson* (2002) Polarization of Cinnamoyl-CoA Substrates Bound to Enoyl-CoA Hydratase: Correlation of 13C NMR with Quantum Mechanical Calculations and Calculation of Electronic Strain Energy Biochemistry 41, 2630-2640.

J. Pawlak, B. J. Bahnson and V. E. Anderson* (2002) DFT/PM3 Study of the enoyl-CoA hydratase catalyzed reaction, Nukleonika, 47 (Supplement 1): 115-155.

Y. H. Pan, B. Z. Yu, A. G. Singer, F. Ghomashchi, G. Lambeau, M. H. Gelb, M. K. Jain, and B. J. Bahnson* (2002) Crystal Structure of Human Group-X Secreted Phospholipase A2; Electrostatically Neutral Interfacial Binding Surface Targets Zwitterionic Membranes,Journal of Biological Chemistry, 277, 29086-29093.

C. Ceccarelli, N. Grodsky, N. Aryaratne, R.F. Colman and B.J. Bahnson* (2002) Crystal Structure of Porcine Mitochondrial NADP+-dependent Isocitrate Dehydrogenase Complexed with Mn2+ and Isocitrate Insights into the Enzyme Mechanism, Journal of Biological Chemistry, 277, 43454-43462.

Y. Pan, B. Z. Yu, O. G. Berg, M. K. Jain and B. J. Bahnson* (2002) Crystal Structure of Phospholipase A2 in Complex with the Hydrolysis Products of Platelet Activating Factor: Equilibrium Binding of Fatty Acid and Lysophospholipid-Ether at the Active Site May Be Mutually Exclusive, Biochemistry, 41, 14790-14800.

P. M. Tessier, H. R. Johnson, R. Pazhianur, B. W. Berger, J. L. Prentice, B. J. Bahnson, S. I. Sandler, and A. M. Lenhoff* (2002) Predictive Crystallization of Ribonuclease A via Rapid Screening of Osmotic Second Virial Coefficients, Proteins: Structure, Function and Genetics, 50, 303-311.

B. P. Smart, Y. H. Pan, A. K. Weeks, J. G. Bollinger, B. J. Bahnson* and M. H. Gelb* (2004) Inhibition of the Complete Set of Mammalian Secreted Phospholipases A2 by Indole Analogs: A Structure-Guided Study, Bioorganic and Medicinal Chemistry, 12, 1737-1749.

C. Ceccarelli, Z. X. Liang, M. Strickler, G. Prehna, B. M. Goldstein, J. P. Klinman, and B. J. Bahnson* (2004) Crystal Structure and Amide H/D Exchange of Binary Complexes of Alcohol Dehydrogenase from B. stearothermophilus: Insights to Thermostability and Cofactor Binding Biochemistry, 43, 5266-5277.

D. T. Yeung, D. Josse, J. D. Nicholson, A. Khanal, C. W. McAndrew, B. J. Bahnson, D. E. Lenz, and D. M. Cerasoli* (2004) Structure/Function Analyses of Human Serum Paraoxonase (HuPON1) Mutants Chosen from a DFPase-like Homology Model Biochim. Biochem. Acta, 1702, 67-77.

B. J. Bahnson* (2004) An atomic-resolution mechanism of 3-hydroxy-3-methylglutaryl-CoA synthase, Proc Natl Acad Sci USA 101, 16399-400.

B. J. Bahnson* (2005) Structure, Function and Interfacial Allosterism in Phospholipase A2: Insight from the Anion-Assisted Dimer, Archives of Biochem. and Biophys., 433, 96-106.

B. Z. Yu, Y. H. Pan, M. Janssen, B. J. Bahnson*, and M. K. Jain* (2005) Kinetic and Structural Properties of Disulfide Engineered Phospholipase A2: Insight into the Role of Disulfide Bonding Patterns, Biochemistry, 44, 3369-79.

C. J. Blamey, C. Ceccarelli, U. P. Naik, and B. J. Bahnson* (2005) The Crystal Structure of Calcium- and Integrin-Binding Protein 1: Insights into Redox Regulated Functions, Protein Science, 14, 1214-1221.

B. Berger, C. J. Blamey, U. P. Naik, B. J. Bahnson, and A. M. Lenhoff* (2005) Roles of Additives and Precipitants in Crystallization of Calcium- and Integrin-Binding Protein, Crystal Growth &  Design, 5, 1499-1507.

J. M. Winget, Y. H. Pan and B. J. Bahnson* (2006) The Interfacial binding surface of phospholipase A2s, Biochim. Biophys. Acta, 1761, 1260-1269, PMID: 16962825.

Y. H. Pan and B. J. Bahnson* (2007) Structural basis for bile salt inhibition of pancreatic phospholipase A2, J. Mol. Biol., 369, 439-450, PMCID: PMC1933606.

U. Samanta and B. J. Bahnson* (2008) Crystal structure of human plasma platelet activating factor acetylhydrolase; Structural implication to lipoprotein binding and catalysis, J. Biol. Chem. 283, 31617-31624, PMCID: PMC2581546.

U. Samanta, C. Wilder, and B. J. Bahnson* (2009) Crystallization and preliminary X-ray diffraction studies of human platelet activating factor acetylhydrolase, Protein & Peptide Letters, 16, 97-100, PMID: 19149681.

T. M. Epstein, U. Samanta, S. D. Kirby, D. M. Cerasoli and B. J. Bahnson* (2009) Crystal structures of brain group-VIII phospholipase A2 in non-aged complexes with the organophosphorus nerve agents soman and sarin, Biochemistry, 48, 3425-3435, PMCID: PMC2671691.

U. Samanta, S. D. Kirby, P. Srinivasan, D. M. Cerasoli and B. J. Bahnson* (2009) Crystal structures of human group-VIIA phospholipase A2 inhibited by organophosphorus nerve agents exhibit non-aged complexes Biochem. Pharmacol., 78, 420-429, PMCID: PMC2759606.

P. Srinivasan and B. J. Bahnson* (2010) Molecular model of plasma PAF acetylhydrolase-lipoprotein association: insights from the structure, Pharmaceuticals 3, 541-557.

Y. H. Pan and B. J. Bahnson* (2010) Structure of a premicellar complex of alkyl sulfates with the interfacial binding surfaces of 4 subunits of phospholipase A2, Biochim. Biophys. Acta, Epub, PMCID: PMC2875363.

S. Chakraborti and B. J. Bahnson* (2010) Crystal structure of human senescence marker protein 30: insights linking structural, enzymatic, and physiological functions, Biochemistry, 49, 3436-3444, PMCID: PMC2871395.

V. N. Daithankar, S. A. Schaefer, M. Dong, B. J. Bahnson*, and C. Thorpe* (2010) Structure of the human sulfhydryl oxidase augmenter of liver regeneration and characterization of a human mutation causing an autosomal recessive myopathy, Biochemistry, 49, 6737-6745.

A. F. Thévenin, C. L Zony, B. J. Bahnson, and R. F. Colman* (2011) Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts, Protein Expression and Purification, 75, 138-46.

A. F. Thévenin, C. L. Zony, B. J. Bahnson, and R. F. Colman* (2011) GST pi modulates JNK activity through a direct interaction with JNK substrate, ATF2, Protein Sci. 2011 May;20(5):834-48.

B. J. Bahnson* (2011) Peripheral membrane protein production for structural and functional studies, in A. S. Robinson, Ed., Production of membrane proteins – strategies for expression and isolation, 1st ed. Weinheim, Germany, Wiley-VCH Verlag.

Z. D. Nagel, M. Dong, B. J. Bahnson, and J. P. Klinman* (2011) Impaired protein conformational landscapes as revealed in anomalous Arrhenius prefactors, Proc Natl Acad Sci U S A. 108, 10520-5.

A. F. Thévenin, E. S. Monillas, J. M. Winget, K. Czymmek, and B. J. Bahnson* (2011) Trafficking of Platelet-activating Factor Acetylhydrolase Type-II in Response to Oxidative Stress, Biochemistry, 50, 8417-8426.

S. H. Scott and B. J. Bahnson* (2011) Senescence marker protein 30: functional and structural insights to its unknown physiological function, Biomolecular Concepts, 2, 469-480.

Z. D. Nagel, C. W. Meadows, M. Dong, B. J. Bahnson, and J. P. Klinman* (2012) Active site hydrophobic residues impact hydrogen tunneling differently in a thermophilic alcohol dehydrogenase at optimal versus non optimal temperatures, Biochemistry, 51, 4147-4156.

S. A. Schaefer, M. Dong, R. P. Rubenstein, W. A. Wilkie, B. J. Bahnson, C. Thorpe, and S. Rozovsky* (2013) (77)Se Enrichment of Proteins Expands the Biological NMR Toolbox, J. Mol. Biol. 425, 222-231.

S. D. Kirby, J. R. Norris, R. J. Smith, B. J. Bahnson, and D. M. Cerasoli* (2013) Human paraoxonase double mutants hydrolyze V and G class organophosphorus nerve agents, Chem. Biol. Interact., 203, 181-185.

J. M. Nagano, K. L. Hsu, L. R. Whitby, M. J. Niphakis, A. E. Speers, S. J. Brown, T. Spicer, V. Fernandez-Vega, J. Ferguson, P. Hodder, P. Srinivasan , T. D. Gonzalez, H. Rosen, B. J. Bahnson, and B. F. Cravatt* (2013) Selective inhibitors and tailored activity probes for lipoprotein-associated phospholipase A(2), Bioorg. Med. Chem. Lett., 23, 839-943.