3D Hydrogel Embedded Microfluidic Systems

In vivo, tissue is highly vascularized to provide nutrients and oxygen necessary for cell survival. Furthermore, complex tissues, such as the liver, contain multiple fluidic systems (cardiovascular, biliary, and lymphatic) that do not directly connect yet interact via biomolecular mass transport. The ability to recapitulate complex in vivo fluidic architecture in synthetic constructs could open new avenues for the implementation of biomimetic microtissues for many in vitro
applications including disease modeling, organ-on-a-chip and human-on-a-chip devices, and high-throughput or patient-specific drug screening models. Toward this goal, we are developing a laser-induced, photocavitation-mediated hydrogel erosion technique to locally degrade synthetic and natural hydrogels in desired 3D configurations for the fabrication of complex 3D microfluidic systems derived from native tissue lymphatic and cardiovascular systems.

Read more about this exciting research!!!

Pradhan et al. “Biofabrication Strategies and Engineered In Vitro Systems for Vascular Mechanobiology.” Advanced Healthcare Materials. 2020.

Guo et al. “Accurate Flow in Augmented Networks (AFAN): an Approach to Generating Three-Dimensional Biomimetic Microfluidic Networks with Controlled Flow.” Analytical Methods. 2019.

Pradhan et al. “Fundamentals of Laser-Based Hydrogel Degradation and Applications in Cell and Tissue Engineering .” Advanced Healthcare Materials. 2017.

Heintz et al. “Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks.” Journal of Visualized Experiments. 2017

Heintz et al. “Fabrication of 3D Biomimetic Microfluidic Networks in Hydrogels.” Advanced Healthcare Materials. 2016