Publications
All Lenhoff group publications are listed below in reverse chronological order. Links connect to Web of Science, which requires an institutional subscription to access. If such access is unavailable, articles may still be searched and obtained through traditional methods. Please note that authentication glitches may also require clicking the article link twice before Web of Science loads correctly.
Publications
2010s
In Situ Characterization of the Microstructural Evolution of Biopharmaceutical Solid-State Formulations with Implications for Protein Stability
Multi-Column Displacement Chromatography for Separation of Charge Variants of Monoclonal Antibodies
DNA Retention on Depth Filters
Contributions of Depth Filter Components to Protein Adsorption in Protein Bioprocessing
Mechanisms of Precipitate Formation during the Purification of an Fc-Fusion Protein
Applications of Proteomic Methods for CHO Host Cell Protein Characterization in Biopharmaceutical Manufacturing
Effects of Resin Architecture and Protein Size on Nanoscale Protein Distribution in Ion-Exchange Media
Koshari, S. H. S., N. J. Wagner and A. M. Lenhoff, Langmuir, 2018. 34: pp. 673-684.
Implications of Turbulent Flow in Connecting Capillaries Used in High Performance Liquid Chromatography
Characterization of Dextran-Grafted Hydrophobic Charge-Induction Resins: Structural Properties, Protein Adsorption and Transport
Ionic Strength-Dependent Changes in Tentacular Ion Exchangers with Variable Ligand Density. II. Functional Properties
Characterization of Protein-Excipient Microheterogeneity in Biopharmaceutical Solid-State Formulations by Confocal Fluorescence Microscopy
Knockout of a Difficult-to-Remove CHO Host Cell Protein, Lipoprotein Lipase, for Improved Polysorbate Stability in Monoclonal Antibody Formulations
Ion-Exchange Chromatography of Proteins: The Inside Story
Lenhoff, A. M., Materials Today Proceedings, 2016. 3: pp. 3559-3567.
Ionic Strength-Dependent Changes in Tentacular Ion Exchangers with Variable Ligand Density. I. Structural Properties
A Critical Examination of the Decoupling Approximation for Small-Angle Scattering from Hard Ellipsoids of Revolution
Characterization of Cross-Linked Cellulosic Ion-Exchange Adsorbents: 2. Protein Sorption and Transport
Determinants of Protein Elution Rates from Preparative Ion-Exchange Adsorbents
Angelo, J. M., and A. M. Lenhoff, Journal of Chromatograohy A, 2016. 1440: pp. 94-104.
Local Crystalline Structure in an Amorphous Protein Dense Phase
Characterization of Lysozyme Adsorption in Cellulosic Chromatographic Materials Using Small-Angle Neutron Scattering
Koshari, S. H. S., N. J. Wagner and A. M. Lenhoff, Journal of Chromatography A, 2015. 1399: 45-52.
Silica-Based Strong Anion Exchange Media for Protein Purification
Expression of Difficult-to-Remove Host Cell Protein Impurities During Extended Chinese Hamster Ovary Cell Culture and Their Impact on Continuous Bioprocessing
A Comparative Study of Monoclonal Antibodies. 1. Phase Behavior and Protein-Protein Interactions
Fluorescence recovery after photobleaching investigation of protein transport and exchange in chromatographic media
Identification and characterization of host cell protein product-associated impurities in monoclonal antibody bioprocessing
Anisotropic Contributions to Protein–Protein Interactions
Recovery of Chinese hamster ovary host cell proteins for proteomic analysis
Polymorphic Protein Crystal Growth: Influence of Hydration and Ions in Glucose Isomerase
Characterization of cross-linked cellulosic ion-exchange adsorbents: 1. Structural properties
Characterization and Suitability of Therapeutic Antibody Dense Phases for Subcutaneous Delivery
Optimization of protein sample preparation for two-dimensional electrophoresis
Modeling of dispersion in a polymeric chromatographic monolith
Insights into Protein Sorption and Desorption on Dextran-Modified Ion-Exchange Media
Protein adsorption and transport in polymer-functionalized ion-exchangers
Protein adsorption and transport in dextran-modified ion-exchange media. III. Effects of resin charge density and dextran content on adsorption and intraparticle uptake
Protein adsorption and transport in dextran-modified ion-exchange media. II. Intraparticle uptake and column breakthrough
Modeling of flow in a polymeric chromatographic monolith
Salting-in characteristics of globular proteins
Shrinking-core modeling of binary chromatographic breakthrough
Hydrophobic interaction chromatography of proteins. IV. Protein adsorption capacity and transport in preparative mode
Self-interaction chromatography of proteins on a microfluidic monolith
Effects of Urea on the Microstructure and Phase Behavior of Aqueous Solutions of Poly(oxyethylene) Surfactants
Interactions and Phase Behavior of a Monoclonal Antibody
Effect of bioparticle size on dispersion and retention in monolithic and perfusive beds
2000s
Protein adsorption and transport in dextran-modified ion-exchange media. I: Adsorption
Comparative Effects of Salt, Organic, and Polymer Precipitants on Protein Phase Behavior and Implications for Vapor Diffusion
Mechanism of formation of lysozyme crystals in concentrated ammonium sulfate solution from concentration profiles and equilibria: Influence of the 2nd osmotic virial coefficient
Relation of structure to performance characteristics of monolithic and perfusive stationary phases
Flow-Dependent Entrapment of Large Bioparticles in Porous Process Media
Binary adsorption of globular proteins on ion-exchange media
Salting-out of lysozyme and ovalbumin from mixtures: Predicting precipitation performance from protein-protein interactions
Effects of pH on protein-protein interactions and implications for protein phase behavior
Protein phase behavior in aqueous solutions: Crystallization, liquid-liquid phase separation, gels, and aggregates
Effects of ammonium sulfate and sodium chloride concentration on PEG/protein liquid-liquid phase separation
Multiscale modeling of protein uptake patterns in chromatographic particles
Binding of alkyl polyglucoside Surfactants to bacteriorhodopsin and its relation to protein stability
Self-interaction nanoparticle spectroscopy: A nanoparticle-based protein interaction assay
Hydrophobic interaction chromatography of proteins III. Transport and kinetic parameters in isocratic elution
A predictive approach to correlating protein adsorption isotherms on ion-exchange media
Solvation free energy of amino acids and side-chain analogues
Patterns of protein – protein interactions in salt solutions and implications for protein crystallization
Chromatography of proteins on charge-variant ion exchangers and implications for optimizing protein uptake rates
Self-assembly of medium-chain alkyl monoglucosides in ammonium sulfate solutions with poly(ethylene glycol)
Hydrophobic interaction chromatography of proteins I. The effects of protein and adsorbent properties on retention and recovery
Hydrophobic interaction chromatography of proteins II. Solution thermodynamic properties as a determinant of retention
Sorption processes in ion-exchange chromatography of viruses
Polyhedral instability of glucose isomerase crystals as revealed by confocal scanning fluorescence microscopy
Nonnative protein polymers: Structure, morphology, and relation to nucleation and growth
Effects of additives on surfactant phase behavior relevant to bacteriorhodopsin crystallization
Kinetics and equilibria of lysozyme precipitation and crystallization in concentrated ammonium sulfate solutions
Effects of pore structure and molecular size on diffusion in chromatographic adsorbents
Three-dimensional pore structure of chromatographic adsorbents from electron tomography
Pore size distributions of ion exchangers and relation to protein binding capacity
Determination of pore size distributions of porous chromatographic adsorbents by inverse size-exclusion chromatography (vol 1037, pg 273, 2004)
A consistent experimental and modeling approach to light-scattering studies of protein-protein interactions in solution
Roles of additives and precipitants in crystallization of calcium- and integrin-binding protein
Relating surfactant properties to activity and solubilization of the human adenosine A(3) receptor
The role of protein and surfactant interactions in membrane-protein crystallization
The combined simulation approach of atomistic and continuum models for the thermodynamics of lysozyme crystals
Effects of ionic strength on lysozyme uptake rates in cation exchangers. I: Uptake in SP sepharose FF
Light-scattering studies of protein solutions: Role of hydration in weak protein-protein interactions
Molecular effects of anionic surfactants on lysozyme precipitation and crystallization
Quantitative imaging by confocal scanning fluorescence microscopy of protein crystallization via liquid-liquid phase separation
Electrostatic contributions to protein retention in ion-exchange chromatography. 2. Proteins with various degrees of structural differences
Determination of fluid-solid transitions in model protein solutions using the histogram reweighting method and expanded ensemble simulations
Effect of spacer arm length on protein retention on a strong cation exchange adsorbent
Relative retention of the fibroblast growth factors FGF-1 and FGF-2 on strong cation-exchange sorbents
Direct measurement of protein osmotic second virial cross coefficients by cross-interaction chromatography
Correlation of diafiltration sieving behavior of lysozyme-BSA mixtures with osmotic second virial cross-coefficients
Electrostatic contributions to protein retention in ion-exchange chromatography. 1. Cytochrome c variants
Determination of pore size distributions of porous chromatographic adsorbents by inverse size-exclusion chromatography
Nondiffusive mechanisms enhance protein uptake rates in ion exchange particles
Casein precipitation equilibria in the presence of calcium ions and phosphates
A natural interaction: Chemical engineering and molecular biophysics
Predictive crystallization of ribonuclease A via rapid screening of osmotic second virial coefficients
Measurements of protein self-association as a guide to crystallization
Measurement of mobility of adsorbed colloids by lateral force microscopy
Mobility of adsorbed proteins studied by fluorescence recovery after photobleaching
On-line spectroscopic characterization of sodium cyanide with nanostructured gold surface-enhanced Raman spectroscopy substrates
Rapid measurement of protein osmotic second virial coefficients by self-interaction chromatography
Self-interaction chromatography: a novel screening method for rational protein crystallization
Effect of pH and Ca2+-induced associations of soybean proteins
Determinants of protein retention characteristics on cation-exchange adsorbents
Effect of gravity on colloidal deposition studied by atomic force microscopy
A patch-antipatch representation of specific protein interactions
Correlation between the osmotic second virial coefficient and the solubility of proteins
Structured metallic films for optical and spectroscopic applications via colloidal crystal templating
Role of competitive interactions in growth rate trends of subtilisin s88 crystals
Calculation of hydration effects in the binding of anionic ligands to basic proteins
Measured and calculated effects of mutations in bacteriophage T4 lysozyme on interactions in solution
Pore size distributions of cation-exchange adsorbents determined by inverse size-exclusion chromatography
Adsorbed layers of ferritin at solid and fluid interfaces studied by atomic force microscopy
Phase equilibria in the lysozyme-ammonium sulfate-water system
Protein crystallization by design: Chymotrypsinogen without precipitants
Assembly of gold nanostructured films templated by colloidal crystals and use in surface-enhanced Raman spectroscopy
Surfactant diffusion into lysozyme crystal matrices investigated by quantitative fluorescence microscopy
Colloidal crystals as templates for porous materials
A class of microstructured particles through colloidal crystallization
Size does matter: electrostatically determined surface coverage trends in protein and colloid adsorption
1990s
Calculation of short-range interactions between proteins
Why is the osmotic second virial coefficient related to protein crystallization?
Protein adsorption isotherms through colloidal energetics
Photochemical micromachining of lysozyme crystals
Materials – A class of porous metallic nanostructures
Characterization of phase separation in mixed surfactant films by liquid tapping mode atomic force microscopy
Comparison of protein adsorption isotherms and uptake rates in preparative cation-exchange materials
Molecular origins of osmotic second virial coefficients of proteins
Electrostatic contribution to the energy and entropy of protein adsorption
Electrical double-layer interaction between heterogeneously charged colloidal particles: A superposition formulation
Microstructured porous silica obtained via colloidal crystal templates
Protein interactions in solution characterized by light and neutron scattering: Comparison of lysozyme and chymotrypsinogen
Influence of structural details in modeling electrostatically driven protein adsorption
Ultracentrifugal crystallization of proteins: transport-kinetic modelling, and experimental behavior of catalase
2-D and 3-D interactions in random sequential adsorption of charged particles
Grand canonical Brownian dynamics simulation of colloidal adsorption
Porous silica via colloidal crystallization
Adsorption of charged latex particles on mica studied by atomic force microscopy
Excluded volume contribution to the osmotic second virial coefficient for proteins (vol 41, pg 1010, 1995)
Diffusion and adsorption of proteins in a model pore in the surface forces apparatus
Improved parametric representation of water dielectric data for Lifshitz theory calculations
Van der Waals interactions involving proteins
Mechanistic model of retention in protein ion-exchange chromatography
Computation of the Electrophoretic Mobility of Proteins
Introduction to Cohn and Edsall Symposium Articles
Excluded-Volume Contribution to the Osmotic 2nd Virial-Coefficient for Proteins
Effect of Taper on Diffusion and Adsorption in a Cylindrical Pore
Molecular Thermodynamic Properties of Protein Solutions from Partial Specific Volumes
Drop Formation in Liquid-Liquid Systems before and after Jetting
Electrostatic and Van-Der-Waals Contributions to Protein Adsorption – Comparison of Theory and Experiment
Electrostatic and Van-Der-Waals Contributions to Protein Adsorption .2. Modeling of Ordered Arrays
Contributions of Surface-Features to the Electrostatic Properties of Rough Colloidal Particles
Energetic and Entropic Contributions to the Interaction of Unequal Spherical Double-Layers
Dynamic Breakup of Liquid-Liquid Jets
Stm and Afm in Biotechnology
Analysis of Ordered Arrays of Adsorbed Lysozyme by Scanning Tunneling Microscopy
Steady Laminar-Flow of Liquid-Liquid Jets at High Reynolds-Numbers
Electrostatic and Vanderwaals Contributions to Protein Adsorption – Computation of Equilibrium-Constants
Consequences of Wall Adsorption in Capillary Electrophoresis – Theory and Simulation
Tirf of Salt and Surface Effects on Protein Adsorption .1. Equilibrium
Tirf of Salt and Surface Effects on Protein Adsorption
Scanning Tunneling Microscopy and Tunneling Spectroscopy of Ordered Heteropolyanion and Isopolyanion Arrays on Graphite
Computation of the Electrostatic Interaction Energy between a Protein and a Charged Surface
Modeling of Particle Separations in Complex Flows
Relation of Protein Electrostatics Computations to Ion-Exchange and Electrophoretic Behavior
Ordered Arrays of Proteins on Graphite Observed by Scanning Tunneling Microscopy
Dispersion in Round Tubes and Its Implications for Extra-Column Dispersion
Dispersion and Partitioning in Short Coated Tubes
Flow in Curved Ducts .2. Rotating Ducts
Partitioning of Host and Recombinant Cells in Aqueous 2-Phase Polymer Systems
A Boundary Element Method for Molecular Electrostatics with Electrolyte Effects
1980s
Affinity Separations Using Liquid Perfluorocarbon Supports – Rate Effects
Flow in Curved Ducts – Bifurcation Structure for Stationary Ducts
Dispersion in Laminar-Flow in Short Tubes
Dispersion Coefficient for Laminar-Flow in Curved Tubes
Significance and Estimation of Chromatographic Parameters