Data analysis of a 96 well HTE plate can become highly complex very quickly. By developing a user-friendly spreadsheet, we can ensure that methods are standardized in our facility. Feel free to use the attached excel sheet to workup your HTE data. Also included in the zip file is a step-by-step pdf on how to utilize the spreadsheet.
Visualizing HTE Data
Crow is a software package for retrieving, diagnosing, and presenting High THroughput Experimentation data from various instruments. Desgined by JAckson Burns at the University of Delaware in the laboratory of Donald Watson in 2019, coded in Python in 2020 and still under active development.
Pie Charts and Heat Mapping
Using Numbers (Mac) we have made an a spreadsheet that generates a 96 or 24 grid of pie charts with with heat mapping behind them. This allows for simple customization of color schemes and easy representation of HTE data.
Sample Submission and Preparation
Vial Sample Submission
Samples for the GC, LC, and SFC can be submitted in the format seen to the left. Any 1.5mL glass vial with a septa top that can be pierced by a needle can be used. Please ensure that all samples are roughly 1mg/mL in concentration. Samples must be filtered before submission, a simple silica plug will suffice.
HTE Plate Submission
HTE plates for the GC, LC, and SFC can be submitted in the format seen to the left. The microtiter plate seen with an aluminum sticker is the only format allowed. If you need a plate of this type please speak to the facility director. Before submission on any instrument the HTE plate must be centrifuged using the 15min method on the Genevac. For help please contact the facility director.