Data analysis of a 96 well HTE plate can become highly complex very quickly. By developing a standardized, user-friendly spreadsheet, we can readily visualize multi-dimensional HTE data in R or Python and long-term ensure that data is machine-readable.
Visualizing HTE Data
Crow is a software package for retrieving, diagnosing, and presenting High Throughput Experimentation data from various instruments. It was designed by Jackson Burns at the University of Delaware in the laboratory of Donald Watson in 2019, coded in Python in 2020, and is still under active development.
Pie Charts and Heat Mapping
Using Numbers (Mac) we have made an a spreadsheet that generates a 96 or 24 grid of pie charts with with heat mapping behind them. This allows for simple customization of color schemes and easy representation of HTE data.
Pie Charts and Donut Plots
Using RStudio we have made an a notebook that generates a grid of donut plots, pie charts, bar charts, or a heat map. These can be customized using standard ggplot commands and written instructions are included in the file. A notebook for compiling exported integration data from Chemstation is also included.
Sample Submission and Preparation
Vial Sample Submission
Samples for the GC-FID, LC-MS, and SFC-MS can be submitted in the format seen to the right. Any 1.5mL glass vial with a pre-slit septa top can be used. Please ensure that all samples have a concentration less than 0.5 mg/mL for LC-MS, SFC-MS, or GC-MS analysis or a concentration of less than 2.5 mg/mL for GC-FID analysis and are filtered before submission. A simple silica plug will suffice and syringe filters are also available in the lab.
HTE Plate Submission
HTE plates for any analytical instrument can be submitted in the format seen to the left. A microtiter plate sealed with a Teflon sticker cover is the only format allowed. Before submission on any instrument the HTE plate must be centrifuged using the 15 min method on the Genevac. If you need a plate of this type please speak to the facility director.