An NIH-designated Center of Biomedical Research Excellence (COBRE)

Multiscale Assessments Research Core

Multimodal Animal Imaging Lab

Mary Boggs, PhD

Dr. Mary Boggs completed her PhD at the University of Delaware in the Department of Biological Sciences under the direction of Dr. Randall Duncan from Biological Sciences and Dr. Tom Beebe, Jr. from the Department of Chemistry and Biochemistry in 2011.  Her graduate work focused on developing bone/nerve cell co-culturing methods and identifying mechanisms of cell-cell communication between these cells.  Post graduation, she stayed on as a Research Associate in the Duncan Lab and was involved in a variety of projects aimed to understand biological molecules involved in bone and cartilage responses to mechanical forces at both the cell and organismal level.  Before joining the DCMR, Dr. Boggs served as the Associate Director of DBI Research at the Delaware Biotechnology Institute.  In this role she provided K-12 STEM education opportunities and training and provided oversight to the shared instrument facilities at the DBI.  Additionally, she served as an Associate Director for the Chemistry/Biology Interface Program where she provided insight and advice to student progress and engaged students in outreach activities and resilience training. As the Lead Scientist for the DCMR, Dr. Boggs is excited to provide her expertise and technical support for those using the DCMR Core Instrumentation.

Lori May

Services offered:

  • Analysis Assistance
  • Assay/Protocol Development
  • Consultation/Training
  • Project Development

Equipment:

Brüker SkyScan 1276 Micro-CT

The SkyScan 1276 is a high performance, stand-alone, fast, desktop in vivo micro-CT with continuously variable magnification for scanning small laboratory animals (mice, rats, hamster, etc.) and biological samples.

Description: The Brüker SkyScan 1276 Micro-CT features a combination of high resolution, big image size, round/spiral scanning, reconstruction, and low dose imaging. The image field of view (up to 80 mm wide and more than 300 mm long) allows full body mouse and rat scanning. The variable magnification allows scanning bone and tissue samples with high spatial resolution down to a 2.8 µm pixel size. Variable X-ray energy combined with a range of filters ensures optimal image quality for diverse research applications from lung tissue to bone with metal implants. Further, the SkyScan 1276 in vivo micro-CT administers low radiation dose to the animals allowing multiple scans in longitudinal pre-clinical studies without the risk of unwanted radiation-induced side effects. The system can perform scanning with continuous gantry rotation and in step-  and-shoot mode with the fastest scanning cycle of 3.9 sec.

The SkyScan 1276 allows reconstructing non-invasively any cross section(s) through the animal body with possibilities to convert reconstructed datasets into realistic 3D-images and to calculate internal morphological parameters including specific bone structural parameters.

 Features:

  • 3.9 second shortest scanning cycle
  • 2.8 micron highest nominal resolution
  • Maintenance-free 20-100 kV micro-focus X-ray source, automatic 6-position filter changer
  • 11 Mp cooled X-ray camera
  • Continuously variable magnification with smallest pixel size of 2.8 micron
  • Possibility to resolve object details of 5-6µm with more than 10% contrast
  • Scanning in circular or spiral (helical) trajectory
  • GPU-accelerated and world’s fastest InstaRecon® 3D reconstruction
  • Full body mouse and rat scanning, 80mm scanning diameter, >300mm scanning length,
  • Step-and-shoot and continuous gantry rotation with shortest scanning cycle of 3.9 sec.
  • Integrated physiological monitoring (breathing, movement detection, ECG) for time-resolved 4D microtomography
  • Software for 2D/3D image analysis, bone morphology and realistic visualization
  • On-screen dose meter to estimate accumulated dose and dose rate
  • Export results to 3D printers to create magnified physical copy of scanned objects

Location: CBBI (Center for Biomedical and Brain Imaging) 244A

Contact person: Dr. Mary Boggs

IVIS Lumina III In Vivo Imaging System

The IVIS ® Lumina Series III brings together years of leading optical imaging technologies into one easy to use and exquisitely sensitive bench-top system. The IVIS Lumina III is capable of imaging both fluorescent and bioluminescent reporters. The system is equipped with up to 26 filter sets that can be used to image reporters that emit from green to near-infrared. Superior spectral unmixing can be achieved by Lumina III’s optional high resolution short cut off filters.

Features:

  • Exquisite sensitivity in bioluminescence
  • Full fluorescence tunability through the NIR spectrum
  • Compute Pure Spectrum spectral unmixing for ultimate fluorescence sensitivity
  • Dedicated XGI-8 Gas Anesthesia System

Location: CBBI (Center for Biomedical and Brain Imaging) 244A

Contact person: Dr. Mary Boggs

Scanco Medicine 35 micro CT

The Scanco 35 microCT system is used to exam diverse materials including bone and other hard tissues as well as medical devices.  This system can be used to assess trabecular bone structure from tibia, femor and vertebrae harvested from rats and mice.