NOTE ONE about PUBLICATIONS:

The CBI Grant is publicly funded by the National Institute of Health, through the National Institute of General Medical Sciences.  Thus, all articles/papers, including their data, tables, figures and supplemental information, must be made publicly available to ensure compliance with the NIH Public Access Policy.

To that end, all papers published in collaboration with a CBI Student need to apply for, and receive, a PMCID number from the National Institute of Health’s Manuscript Submission System (NIHMS.)

For further information click HERE.

NOTE TWO about PUBLICATIONS:

Each publication, press release, or other document about research supported by an NIH award must include an acknowledgment of NIH award support and a disclaimer such as “Research reported in this publication was supported by the National Institute Of General Medical Sciences of the National Institutes of Health under Award Number T32GM133395. The content is solely the responsibility of the authors and does not necessarily represent the  official views of the National Institutes of Health.”
 
Prior to issuing a press release concerning the outcome of this research, please notify the NIH awarding IC in advance to allow for coordination

2020

Berckman, Emily A., Emily J. Hartzell, Alexander A. Mitkas, Qing Sun, and Wilfred Chen. “Biological Assembly of Modular Protein Building Blocks as Sensing, Delivery, and Therapeutic Agents.” Annual Review of Chemical and Biomolecular Engineering 11, no. 1 (June 7, 2020): 35–62. https://doi.org/10.1146/annurev-chembioeng-101519-121526.

Irvin-Choy, N’Dea S., Katherine M. Nelson, Jason P. Gleghorn, and Emily S. Day. “Design of Nanomaterials for Applications in Maternal/Fetal Medicine.” Journal of Materials Chemistry B, 2020, 10.1039.D0TB00612B. https://doi.org/10.1039/D0TB00612B.

Shirazi, Jasmine, Michael J. Donzanti, Katherine M. Nelson, Ryan Zurakowski, Catherine A. Fromen, and Jason P. Gleghorn. “Significant Unresolved Questions and Opportunities for Bioengineering in Understanding and Treating COVID-19 Disease Progression.” Cellular and Molecular Bioengineering, July 27, 2020. https://doi.org/10.1007/s12195-020-00637-w.

Ukaegbu, Ophelia I., Kristen E. DeMeester, Hai Liang, Ashley R. Brown, Zachary S. Jones, and Catherine Leimkuhler Grimes. “Utility of Bacterial Peptidoglycan Recycling Enzymes in the Chemoenzymatic Synthesis of Valuable UDP Sugar Substrates.” In Methods in Enzymology, 638:1–26. Elsevier, 2020. https://doi.org/10.1016/bs.mie.2020.02.014.

Valcourt, Danielle M., Megan N. Dang, Mackenzie A. Scully, and Emily S. Day. “Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer.” ACS Nano 14, no. 3 (March 24, 2020): 3378–88. https://doi.org/10.1021/acsnano.9b09263.

Valcourt, Danielle M., Chintan H. Kapadia, Mackenzie A. Scully, Megan N. Dang, and Emily S. Day. “Best Practices for Preclinical In Vivo Testing of Cancer Nanomedicines.” Advanced Healthcare Materials 9, no. 12 (June 2020): 2000110. https://doi.org/10.1002/adhm.202000110.

Wang, Jianxin, Megan N. Dang, and Emily S. Day. “Inhibition of Wnt Signaling by Frizzled7 Antibody-Coated Nanoshells Sensitizes Triple-Negative Breast Cancer Cells to the Autophagy Regulator Chloroquine.” Nano Research 13, no. 6 (June 2020): 1693–1703. https://doi.org/10.1007/s12274-020-2795-8.

Wodzanowski, Kimberly A., Samantha E. Cassel, Catherine L. Grimes, and April M. Kloxin. “Tools for Probing Host-Bacteria Interactions in the Gut Microenvironment: From Molecular to Cellular Levels.” Bioorganic & Medicinal Chemistry Letters 30, no. 10 (May 2020): 127116. https://doi.org/10.1016/j.bmcl.2020.127116.

2019

(1)  D’Ambrosio, Elizabeth A., Walter R. Drake, Siavash Mashayekh, Ophelia I. Ukaegbu, Ashley R.  Brown, and Catherine L. Grimes. “Modulation of the NOD-like Receptors NOD1 and NOD2: A Chemist’s Perspective.” Bioorganic & Medicinal Chemistry Letters 29, no. 10 (May 15, 2019): 1153–61. https://doi.org/10.1016/j.bmcl.2019.03.010.

(2)  DeMeester, Kristen E., Hai Liang, Junhui Zhou, Kimberly A. Wodzanowski, Benjamin L. Prather, Cintia C. Santiago, and Catherine L. Grimes. “Metabolic Incorporation of N ‐Acetyl Muramic Acid Probes into Bacterial Peptidoglycan.” Current Protocols in Chemical Biology 11, no. 4 (December 2019). https://doi.org/10.1002/cpch.74.

(3)  Fritz, Matthew, Jodi Kraus, Caitlin M. Quinn, Glenn P. A. Yap, Jochem Struppe, Ivan V. Sergeyev, Angela M. Gronenborn, and Tatyana Polenova. “Measurement of Accurate Interfluorine Distances in Crystalline Organic Solids: A High-Frequency Magic Angle Spinning NMR Approach.” The Journal of Physical Chemistry B, December 10, 2019, acs.jpcb.9b08919. https://doi.org/10.1021/acs.jpcb.9b08919.

(4)  Lazor, Klare M., Junhui Zhou, Kristen E. DeMeester, Elizabeth A. D’Ambrosio, and Catherine L. Grimes. “Synthesis and Application of Methyl N,O-Hydroxylamine Muramyl Peptides.” Chembiochem: A European Journal of Chemical Biology, January 22, 2019. https://doi.org/10.1002/cbic.201800731.

(5)  M. Pike, Colleen, Rebecca R. Noll, and M. Ramona Neunuebel. “Exploitation of Phosphoinositides by the Intracellular Pathogen, Legionella Pneumophila.” In Pathogenic Bacteria [Working Title]. IntechOpen, 2019. https://doi.org/10.5772/intechopen.89158.

(6)  McDonald, Nathan D., Abish Regmi, Daniel P. Morreale, Joseph D. Borowski, and E. Fidelma Boyd. “CRISPR-Cas Systems Are Present Predominantly on Mobile Genetic Elements in Vibrio Species.” BMC Genomics 20, no. 1 (December 2019). https://doi.org/10.1186/s12864-019-5439-1.

(7)  Millar-Haskell, Catherine S., Allyson M. Dang, and Jason P. Gleghorn. “Coupling Synthetic Biology and Programmable Materials to Construct Complex Tissue Ecosystems.” MRS Communications 9, no. 02 (June 2019): 421–32. https://doi.org/10.1557/mrc.2019.69.

(8)  Munsell, Erik V., Bing Fang, and Millicent O. Sullivan. “Histone-Mimetic Gold Nanoparticles as Versatile Scaffolds for Gene Transfer and Chromatin Analysis.” Bioconjugate Chemistry 29, no. 11 (November 21, 2018): 3691–3704. https://doi.org/10.1021/acs.bioconjchem.8b00611.

(9)  Prhashanna, Ammu, Phillip A. Taylor, Jingya Qin, Kristi L. Kiick, and Arthi Jayaraman. “Effect of Peptide Sequence on the LCST-Like Transition of Elastin-Like Peptides and Elastin-Like Peptide–Collagen-Like Peptide Conjugates: Simulations and Experiments.” Biomacromolecules 20, no. 3 (March 11, 2019): 1178–89. https://doi.org/10.1021/acs.biomac.8b01503.

(10) Russell, Ryan W., Matthew P. Fritz, Jodi Kraus, Caitlin M. Quinn, Tatyana Polenova, and Angela M. Gronenborn. “Accuracy and Precision of Protein Structures Determined by Magic Angle Spinning NMR Spectroscopy: For Some ‘with a Little Help from a Friend.’” Journal of Biomolecular NMR 73, no. 6–7 (July 2019): 333–46. https://doi.org/10.1007/s10858-019-00233-9.

(11) Zhang, Yuhao, Cristian Coarfa, Xiaoyu Dong, Weiwu Jiang, Brielle Hayward-Piatkovskyi, Jason P. Gleghorn, and Krithika Lingappan. “MicroRNA-30a as a Candidate Underlying Sex-Specific Differences in Neonatal Hyperoxic Lung Injury: Implications for BPD.” American Journal of Physiology-Lung Cellular and Molecular Physiology 316, no. 1 (January 2019): L144–56. https://doi.org/10.1152/ajplung.00372.2018.

2018

(1)DeMeester, K. E.; Liang, H.; Jensen, M. R.; Jones, Z. S.; D’Ambrosio, E. A.; Scinto, S. L.; Zhou, J.; Grimes, C. L. Synthesis of Functionalized N-Acetyl Muramic Acids To Probe Bacterial Cell Wall Recycling and Biosynthesis. J. Am. Chem. Soc. 2018, 140 (30), 9458–9465. https://doi.org/10.1021/jacs.8b03304.

(2)DeWaal, D.; Nogueira, V.; Terry, A. R.; Patra, K. C.; Jeon, S.-M.; Guzman, G.; Au, J.; Long, C. P.; Antoniewicz, M. R.; Hay, N. Hexokinase-2 Depletion Inhibits Glycolysis and Induces Oxidative Phosphorylation in Hepatocellular Carcinoma and Sensitizes to Metformin. Nat Commun 2018, 9 (1), 446. https://doi.org/10.1038/s41467-017-02733-4.

(3)DeWaal, D.; Nogueira, V.; Terry, A. R.; Patra, K. C.; Jeon, S.-M.; Guzman, G.; Au, J.; Long, C. P.; Antoniewicz, M. R.; Hay, N. Author Correction: Hexokinase-2 Depletion Inhibits Glycolysis and Induces Oxidative Phosphorylation in Hepatocellular Carcinoma and Sensitizes to Metformin. Nat Commun 2018, 9 (1), 2539. https://doi.org/10.1038/s41467-018-04182-z.

(4)Drake, W. R.; Hou, C.-W.; Zachara, N. E.; Grimes, C. L. New Use for CETSA: Monitoring Innate Immune Receptor Stability via Post-Translational Modification by OGT. J. Bioenerg. Biomembr. 2018, 50 (3), 231–240. https://doi.org/10.1007/s10863-018-9754-z.

(5)Fritz, M.; Quinn, C. M.; Wang, M.; Hou, G.; Lu, X.; Koharudin, L. M. I.; Struppe, J.; Case, D. A.; Polenova, T.; Gronenborn, A. M. Determination of Accurate Backbone Chemical Shift Tensors in Microcrystalline Proteins by Integrating MAS NMR and QM/MM. Phys Chem Chem Phys 2018, 20 (14), 9543–9553. https://doi.org/10.1039/c8cp00647d.

(6)Hamaker, N. K.; Lee, K. H. Site-Specific Integration Ushers in a New Era of Precise CHO Cell Line Engineering. Current Opinion in Chemical Engineering 2018, 22, 152–160. https://doi.org/10.1016/j.coche.2018.09.011.

(7)Hudson, D. A.; Caplan, J. L.; Thorpe, C. Designing Flavoprotein-GFP Fusion Probes for Analyte-Specific Ratiometric Fluorescence Imaging. Biochemistry 2018, 57 (7), 1178–1189. https://doi.org/10.1021/acs.biochem.7b01132.

(8)Jones, A. J.; Fast, A. G.; Clupper, M.; Papoutsakis, E. T. Small and Low but Potent: The Complex Regulatory Role of the Small RNA SolB in Solventogenesis in Clostridium Acetobutylicum. Appl. Environ. Microbiol. 2018, 84 (14). https://doi.org/10.1128/AEM.00597-18.

(9)Kraus, J.; Gupta, R.; Yehl, J.; Lu, M.; Case, D. A.; Gronenborn, A. M.; Akke, M.; Polenova, T. Chemical Shifts of the Carbohydrate Binding Domain of Galectin-3 from Magic Angle Spinning NMR and Hybrid Quantum Mechanics/Molecular Mechanics Calculations. J Phys Chem B 2018, 122 (11), 2931–2939. https://doi.org/10.1021/acs.jpcb.8b00853.

(10)Liu, J.; Chen, Q.; Rozovsky, S. Selenocysteine-Mediated Expressed Protein Ligation of SELENOM. Methods Mol. Biol. 2018, 1661, 265–283. https://doi.org/10.1007/978-1-4939-7258-6_19.

(11)Long, C. P.; Antoniewicz, M. R. How Adaptive Evolution Reshapes Metabolism to Improve Fitness: Recent Advances and Future Outlook. Current Opinion in Chemical Engineering 2018, 22, 209–215. https://doi.org/10.1016/j.coche.2018.11.001.

(12)Long, C. P.; Gonzalez, J. E.; Feist, A. M.; Palsson, B. O.; Antoniewicz, M. R. Dissecting the Genetic and Metabolic Mechanisms of Adaptation to the Knockout of a Major Metabolic Enzyme in Escherichia Coli. Proc. Natl. Acad. Sci. U.S.A. 2018, 115 (1), 222–227. https://doi.org/10.1073/pnas.1716056115.

(13)Lu, M.; Sarkar, S.; Wang, M.; Kraus, J.; Fritz, M.; Quinn, C. M.; Bai, S.; Holmes, S. T.; Dybowski, C.; Yap, G. P. A.; et al. 19F Magic Angle Spinning NMR Spectroscopy and Density Functional Theory Calculations of Fluorosubstituted Tryptophans: Integrating Experiment and Theory for Accurate Determination of Chemical Shift Tensors. J Phys Chem B 2018, 122 (23), 6148–6155. https://doi.org/10.1021/acs.jpcb.8b00377.

(14)MacDonald, M. L.; Hamaker, N. K.; Lee, K. H. Bioinformatic Analysis of Chinese Hamster Ovary Host Cell Protein Lipases. AIChE Journal 2018, 64 (12), 4247–4254. https://doi.org/10.1002/aic.16378.

(15)McDonald, N. D.; DeMeester, K. E.; Lewis, A. L.; Grimes, C. L.; Boyd, E. F. Structural and Functional Characterization of a Modified Legionaminic Acid Involved in Glycosylation of a Bacterial Lipopolysaccharide. J. Biol. Chem. 2018, 293 (49), 19113–19126. https://doi.org/10.1074/jbc.RA118.004966.

(16)Munsell, E. V.; Fang, B.; Sullivan, M. O. Histone-Mimetic Gold Nanoparticles as Versatile Scaffolds for Gene Transfer and Chromatin Analysis. Bioconjug. Chem. 2018, 29 (11), 3691–3704. https://doi.org/10.1021/acs.bioconjchem.8b00611.

(17)Munsell, E. V.; Kurpad, D. S.; Freeman, T. A.; Sullivan, M. O. Histone-Targeted Gene Transfer of Bone Morphogenetic Protein-2 Enhances Mesenchymal Stem Cell Chondrogenic Differentiation. Acta Biomater 2018, 71, 156–167. https://doi.org/10.1016/j.actbio.2018.02.021.

(18)O’Brien, J. G. K.; Chintala, S. R.; Fox, J. M. Stereoselective Synthesis of Bicyclo[6.1.0]Nonene Precursors of the Bioorthogonal Reagents s-TCO and BCN. J. Org. Chem. 2018, 83 (14), 7500–7503. https://doi.org/10.1021/acs.joc.7b02329.

(19)Quinn, C. M.; Wang, M.; Fritz, M. P.; Runge, B.; Ahn, J.; Xu, C.; Perilla, J. R.; Gronenborn, A. M.; Polenova, T. Dynamic Regulation of HIV-1 Capsid Interaction with the Restriction Factor TRIM5α Identified by Magic-Angle Spinning NMR and Molecular Dynamics Simulations. Proc. Natl. Acad. Sci. U.S.A. 2018, 115 (45), 11519–11524. https://doi.org/10.1073/pnas.1800796115.

(20)Wang, M.; Lu, M.; Fritz, M. P.; Quinn, C. M.; Byeon, I.-J. L.; Byeon, C.-H.; Struppe, J.; Maas, W.; Gronenborn, A. M.; Polenova, T. Fast Magic-Angle Spinning 19 F NMR Spectroscopy of HIV-1 Capsid Protein Assemblies. Angew. Chem. Int. Ed. Engl. 2018, 57 (50), 16375–16379. https://doi.org/10.1002/anie.201809060.

(21)Wolfsberg, E.; Long, C. P.; Antoniewicz, M. R. Metabolism in Dense Microbial Colonies: 13C Metabolic Flux Analysis of E. Coli Grown on Agar Identifies Two Distinct Cell Populations with Acetate Cross-Feeding. Metabolic Engineering 2018, 49, 242–247. https://doi.org/10.1016/j.ymben.2018.08.013.

(22)Yu, X.; Noll, R. R.; Romero Dueñas, B. P.; Allgood, S. C.; Barker, K.; Caplan, J. L.; Machner, M. P.; LaBaer, J.; Qiu, J.; Neunuebel, M. R. Legionella Effector AnkX Interacts with Host Nuclear Protein PLEKHN1. BMC Microbiol. 2018, 18 (1), 5. https://doi.org/10.1186/s12866-017-1147-7.

2017

(1)Allgood, S. C.; Romero Dueñas, B. P.; Noll, R. R.; Pike, C.; Lein, S.; Neunuebel, M. R. Legionella Effector AnkX Disrupts Host Cell Endocytic Recycling in a Phosphocholination-Dependent Manner. Front Cell Infect Microbiol 2017, 7, 397. https://doi.org/10.3389/fcimb.2017.00397.

(2)Chiu, J.; Valente, K. N.; Levy, N. E.; Min, L.; Lenhoff, A. M.; Lee, K. H. Knockout of a Difficult-to-Remove CHO Host Cell Protein, Lipoprotein Lipase, for Improved Polysorbate Stability in Monoclonal Antibody Formulations. Biotechnol. Bioeng. 2017, 114 (5), 1006–1015. https://doi.org/10.1002/bit.26237.

(3)Cordova, L. T.; Cipolla, R. M.; Swarup, A.; Long, C. P.; Antoniewicz, M. R. 13C Metabolic Flux Analysis of Three Divergent Extremely Thermophilic Bacteria: Geobacillus Sp. LC300, Thermus Thermophilus HB8, and Rhodothermus Marinus DSM 4252. Metab. Eng. 2017, 44, 182–190. https://doi.org/10.1016/j.ymben.2017.10.007.

(4)Fritz, M.; Quinn, C. M.; Wang, M.; Hou, G.; Lu, X.; Koharudin, L. M. I.; Polenova, T.; Gronenborn, A. M. Toward Closing the Gap: Quantum Mechanical Calculations and Experimentally Measured Chemical Shifts of a Microcrystalline Lectin. J Phys Chem B 2017, 121 (15), 3574–3585. https://doi.org/10.1021/acs.jpcb.6b09479.

(5)Gaynor, A. S.; Chen, W. Induced Prodrug Activation by Conditional Protein Degradation. J. Biotechnol. 2017, 260, 62–66. https://doi.org/10.1016/j.jbiotec.2017.09.005.

(6)Gonzalez, J. E.; Long, C. P.; Antoniewicz, M. R. Comprehensive Analysis of Glucose and Xylose Metabolism in Escherichia Coli under Aerobic and Anaerobic Conditions by 13C Metabolic Flux Analysis. Metab. Eng. 2017, 39, 9–18. https://doi.org/10.1016/j.ymben.2016.11.003.

(7)Lauro, M. L.; D’Ambrosio, E. A.; Bahnson, B. J.; Grimes, C. L. Molecular Recognition of Muramyl Dipeptide Occurs in the Leucine-Rich Repeat Domain of Nod2. ACS Infect Dis 2017, 3 (4), 264–270. https://doi.org/10.1021/acsinfecdis.6b00154.

(8)Liang, H.; DeMeester, K. E.; Hou, C.-W.; Parent, M. A.; Caplan, J. L.; Grimes, C. L. Metabolic Labelling of the Carbohydrate Core in Bacterial Peptidoglycan and Its Applications. Nat Commun 2017, 8, 15015. https://doi.org/10.1038/ncomms15015.

(9)Liu, J.; Chen, Q.; Rozovsky, S. Utilizing Selenocysteine for Expressed Protein Ligation and Bioconjugations. J. Am. Chem. Soc. 2017, 139 (9), 3430–3437. https://doi.org/10.1021/jacs.6b10991.

(10)Long, C. P.; Au, J.; Sandoval, N. R.; Gebreselassie, N. A.; Antoniewicz, M. R. Enzyme I Facilitates Reverse Flux from Pyruvate to Phosphoenolpyruvate in Escherichia Coli. Nat Commun 2017, 8, 14316. https://doi.org/10.1038/ncomms14316.

(11)Long, C. P.; Gonzalez, J. E.; Cipolla, R. M.; Antoniewicz, M. R. Metabolism of the Fast-Growing Bacterium Vibrio Natriegens Elucidated by 13C Metabolic Flux Analysis. Metab. Eng. 2017, 44, 191–197. https://doi.org/10.1016/j.ymben.2017.10.008.

(12)Long, C. P.; Gonzalez, J. E.; Feist, A. M.; Palsson, B. O.; Antoniewicz, M. R. Fast Growth Phenotype of E. Coli K-12 from Adaptive Laboratory Evolution Does Not Require Intracellular Flux Rewiring. Metab. Eng. 2017, 44, 100–107. https://doi.org/10.1016/j.ymben.2017.09.012.

(13)Luo, T.; David, M. A.; Dunshee, L. C.; Scott, R. A.; Urello, M. A.; Price, C.; Kiick, K. L. Thermoresponsive Elastin-b-Collagen-Like Peptide Bioconjugate Nanovesicles for Targeted Drug Delivery to Collagen-Containing Matrices. Biomacromolecules 2017, 18 (8), 2539–2551. https://doi.org/10.1021/acs.biomac.7b00686.

(14)McNeely, P. M.; Naranjo, A. N.; Forsten-Williams, K.; Robinson, A. S. A2AR Binding Kinetics in the Ligand Depletion Regime. SLAS Discov 2017, 22 (2), 166–175. https://doi.org/10.1177/1087057116667256.

(15)Ott, C. A.; Baljinnyam, B.; Zakharov, A. V.; Jadhav, A.; Simeonov, A.; Zhuang, Z. Cell Lysate-Based AlphaLISA Deubiquitinase Assay Platform for Identification of Small Molecule Inhibitors. ACS Chem. Biol. 2017, 12 (9), 2399–2407. https://doi.org/10.1021/acschembio.7b00543.

(16)Rohlhill, J.; Sandoval, N. R.; Papoutsakis, E. T. Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia Coli Growth on Methanol. ACS Synth Biol 2017, 6 (8), 1584–1595. https://doi.org/10.1021/acssynbio.7b00114.

(17)Schaefer, A. K.; Melnyk, J. E.; Baksh, M. M.; Lazor, K. M.; Finn, M. G.; Grimes, C. L. Membrane Association Dictates Ligand Specificity for the Innate Immune Receptor NOD2. ACS Chem. Biol. 2017, 12 (8), 2216–2224. https://doi.org/10.1021/acschembio.7b00469.

(18)Schaefer, A. K.; Wastyk, H. C.; Mohanan, V.; Hou, C.-W.; Lauro, M. L.; Melnyk, J. E.; Burch, J. M.; Grimes, C. L. Crohn’s Disease Variants of Nod2 Are Stabilized by the Critical Contact Region of Hsp70. Biochemistry 2017, 56 (34), 4445–4448. https://doi.org/10.1021/acs.biochem.7b00470.

(19)Struppe, J.; Quinn, C. M.; Lu, M.; Wang, M.; Hou, G.; Lu, X.; Kraus, J.; Andreas, L. B.; Stanek, J.; Lalli, D.; et al. Expanding the Horizons for Structural Analysis of Fully Protonated Protein Assemblies by NMR Spectroscopy at MAS Frequencies above 100 KHz. Solid State Nucl Magn Reson 2017, 87, 117–125. https://doi.org/10.1016/j.ssnmr.2017.07.001.

(20)Urello, M. A.; Kiick, K. L.; Sullivan, M. O. ECM Turnover-Stimulated Gene Delivery through Collagen-Mimetic Peptide-Plasmid Integration in Collagen. Acta Biomater 2017, 62, 167–178. https://doi.org/10.1016/j.actbio.2017.08.038.

(21)Wang, Y.; Lazor, K. M.; DeMeester, K. E.; Liang, H.; Heiss, T. K.; Grimes, C. L. Postsynthetic Modification of Bacterial Peptidoglycan Using Bioorthogonal N-Acetylcysteamine Analogs and Peptidoglycan O-Acetyltransferase B. J. Am. Chem. Soc. 2017, 139 (39), 13596–13599. https://doi.org/10.1021/jacs.7b06820.

2016

(1)Ahn, W. S.; Crown, S. B.; Antoniewicz, M. R. Evidence for Transketolase-like TKTL1 Flux in CHO Cells Based on Parallel Labeling Experiments and (13)C-Metabolic Flux Analysis. Metab. Eng. 2016, 37, 72–78. https://doi.org/10.1016/j.ymben.2016.05.005.

(2)Baeyens, N.; Larrivée, B.; Ola, R.; Hayward-Piatkowskyi, B.; Dubrac, A.; Huang, B.; Ross, T. D.; Coon, B. G.; Min, E.; Tsarfati, M.; et al. Defective Fluid Shear Stress Mechanotransduction Mediates Hereditary Hemorrhagic Telangiectasia. J. Cell Biol. 2016, 214 (7), 807–816. https://doi.org/10.1083/jcb.201603106.

(3)Bascom, C. S.; Wu, S.-Z.; Nelson, K.; Oakey, J.; Bezanilla, M. Long-Term Growth of Moss in Microfluidic Devices Enables Subcellular Studies in Development. Plant Physiol. 2016, 172 (1), 28–37. https://doi.org/10.1104/pp.16.00879.

(4)Cordova, L. T.; Lu, J.; Cipolla, R. M.; Sandoval, N. R.; Long, C. P.; Antoniewicz, M. R. Co-Utilization of Glucose and Xylose by Evolved Thermus Thermophilus LC113 Strain Elucidated by (13)C Metabolic Flux Analysis and Whole Genome Sequencing. Metab. Eng. 2016, 37, 63–71. https://doi.org/10.1016/j.ymben.2016.05.001.

(5)Crown, S. B.; Kelleher, J. K.; Rouf, R.; Muoio, D. M.; Antoniewicz, M. R. Comprehensive Metabolic Modeling of Multiple 13C-Isotopomer Data Sets to Study Metabolism in Perfused Working Hearts. Am. J. Physiol. Heart Circ. Physiol. 2016, 311 (4), H881–H891. https://doi.org/10.1152/ajpheart.00428.2016.

(6)Crown, S. B.; Long, C. P.; Antoniewicz, M. R. Optimal Tracers for Parallel Labeling Experiments and 13C Metabolic Flux Analysis: A New Precision and Synergy Scoring System. Metab. Eng. 2016, 38, 10–18. https://doi.org/10.1016/j.ymben.2016.06.001.

(7)DeAngelis, A.; Panish, R.; Fox, J. M. Rh-Catalyzed Intermolecular Reactions of α-Alkyl-α-Diazo Carbonyl Compounds with Selectivity over β-Hydride Migration. Acc. Chem. Res. 2016, 49 (1), 115–127. https://doi.org/10.1021/acs.accounts.5b00425.

(8)Fu, J.; Sun, F.; Liu, W.; Liu, Y.; Gedam, M.; Hu, Q.; Fridley, C.; Quigley, H. A.; Hanes, J.; Pitha, I. Subconjunctival Delivery of Dorzolamide-Loaded Poly(Ether-Anhydride) Microparticles Produces Sustained Lowering of Intraocular Pressure in Rabbits. Mol. Pharm. 2016, 13 (9), 2987–2995. https://doi.org/10.1021/acs.molpharmaceut.6b00343.

(9)Hilderbrand, A. M.; Ovadia, E. M.; Rehmann, M. S.; Kharkar, P. M.; Guo, C.; Kloxin, A. M. Biomaterials for 4D Stem Cell Culture. Curr Opin Solid State Mater Sci 2016, 20 (4), 212–224. https://doi.org/10.1016/j.cossms.2016.03.002.

(10)Hou, C.-W.; Lauro, M. L.; Leimkuhler Grimes, C. Redefining the Defensive Line: Critical Components of the Innate Immune System. ACS Infect Dis 2016, 2 (11), 746–748. https://doi.org/10.1021/acsinfecdis.6b00174.

(11)Kharkar, P. M.; Rehmann, M. S.; Skeens, K. M.; Maverakis, E.; Kloxin, A. M. Thiol-Ene Click Hydrogels for Therapeutic Delivery. ACS Biomater Sci Eng 2016, 2 (2), 165–179. https://doi.org/10.1021/acsbiomaterials.5b00420.

(12)Lauro, M. L.; Burch, J. M.; Grimes, C. L. The Effect of NOD2 on the Microbiota in Crohn’s Disease. Curr. Opin. Biotechnol. 2016, 40, 97–102. https://doi.org/10.1016/j.copbio.2016.02.028.

(13)Long, C. P.; Au, J.; Gonzalez, J. E.; Antoniewicz, M. R. 13C Metabolic Flux Analysis of Microbial and Mammalian Systems Is Enhanced with GC-MS Measurements of Glycogen and RNA Labeling. Metab. Eng. 2016, 38, 65–72. https://doi.org/10.1016/j.ymben.2016.06.007.

(14)Long, C. P.; Gonzalez, J. E.; Sandoval, N. R.; Antoniewicz, M. R. Characterization of Physiological Responses to 22 Gene Knockouts in Escherichia Coli Central Carbon Metabolism. Metab. Eng. 2016, 37, 102–113. https://doi.org/10.1016/j.ymben.2016.05.006.

(15)McDonald, N. D.; Lubin, J.-B.; Chowdhury, N.; Boyd, E. F. Host-Derived Sialic Acids Are an Important Nutrient Source Required for Optimal Bacterial Fitness In Vivo. MBio 2016, 7 (2), e02237-02215. https://doi.org/10.1128/mBio.02237-15.

(16)Munsell, E. V.; Ross, N. L.; Sullivan, M. O. Journey to the Center of the Cell: Current Nanocarrier Design Strategies Targeting Biopharmaceuticals to the Cytoplasm and Nucleus. Curr. Pharm. Des. 2016, 22 (9), 1227–1244.

(17)Panish, R. A.; Chintala, S. R.; Fox, J. M. A Mixed-Ligand Chiral Rhodium(II) Catalyst Enables the Enantioselective Total Synthesis of Piperarborenine B. Angew. Chem. Int. Ed. Engl. 2016, 55 (16), 4983–4987. https://doi.org/10.1002/anie.201600766.

(18)Raeeszadeh-Sarmazdeh, M.; Hartzell, E.; Price, J. V.; Chen, W. Protein Nanoparticles as Multifunctional Biocatalysts and Health Assessment Sensors. Current Opinion in Chemical Engineering 2016, 13, 109–118. https://doi.org/10.1016/j.coche.2016.08.016.

(19)Rehmann, M. S.; Luna, J. I.; Maverakis, E.; Kloxin, A. M. Tuning Microenvironment Modulus and Biochemical Composition Promotes Human Mesenchymal Stem Cell Tenogenic Differentiation. J Biomed Mater Res A 2016, 104 (5), 1162–1174. https://doi.org/10.1002/jbm.a.35650.

(20)Sandberg, T. E.; Long, C. P.; Gonzalez, J. E.; Feist, A. M.; Antoniewicz, M. R.; Palsson, B. O. Evolution of E. Coli on [U-13C]Glucose Reveals a Negligible Isotopic Influence on Metabolism and Physiology. PLoS ONE 2016, 11 (3), e0151130. https://doi.org/10.1371/journal.pone.0151130.

(21)Urello, M. A.; Kiick, K. L.; Sullivan, M. O. Integration of Growth Factor Gene Delivery with Collagen-Triggered Wound Repair Cascades Using Collagen-Mimetic Peptides. Bioeng Transl Med 2016, 1 (2), 207–219. https://doi.org/10.1002/btm2.10037.

(22)Yao, M.; Elling, F. J.; Jones, C.; Nomosatryo, S.; Long, C. P.; Crowe, S. A.; Antoniewicz, M. R.; Hinrichs, K.-U.; Maresca, J. A. Heterotrophic Bacteria from an Extremely Phosphate-Poor Lake Have Conditionally Reduced Phosphorus Demand and Utilize Diverse Sources of Phosphorus. Environ. Microbiol. 2016, 18 (2), 656–667. https://doi.org/10.1111/1462-2920.13063.

(23)Zhang, H.; Trout, W. S.; Liu, S.; Andrade, G. A.; Hudson, D. A.; Scinto, S. L.; Dicker, K. T.; Li, Y.; Lazouski, N.; Rosenthal, J.; et al. Rapid Bioorthogonal Chemistry Turn-on through Enzymatic or Long Wavelength Photocatalytic Activation of Tetrazine Ligation. J. Am. Chem. Soc. 2016, 138 (18), 5978–5983. https://doi.org/10.1021/jacs.6b02168.

(24)Zuñiga, C.; Li, C.-T.; Huelsman, T.; Levering, J.; Zielinski, D. C.; McConnell, B. O.; Long, C. P.; Knoshaug, E. P.; Guarnieri, M. T.; Antoniewicz, M. R.; et al. Genome-Scale Metabolic Model for the Green Alga Chlorella Vulgaris UTEX 395 Accurately Predicts Phenotypes under Autotrophic, Heterotrophic, and Mixotrophic Growth Conditions. Plant Physiol. 2016, 172 (1), 589–602. https://doi.org/10.1104/pp.16.00593.

2015

(1)Boyd, E. F.; Carpenter, M. R.; Chowdhury, N.; Cohen, A. L.; Haines-Menges, B. L.; Kalburge, S. S.; Kingston, J. J.; Lubin, J. B.; Ongagna-Yhombi, S. Y.; Whitaker, W. B. Post-Genomic Analysis of Members of the Family Vibrionaceae. Microbiol Spectr 2015, 3 (5). https://doi.org/10.1128/microbiolspec.VE-0009-2014.

(2)Cordova, L. T.; Long, C. P.; Venkataramanan, K. P.; Antoniewicz, M. R. Complete Genome Sequence, Metabolic Model Construction and Phenotypic Characterization of Geobacillus LC300, an Extremely Thermophilic, Fast Growing, Xylose-Utilizing Bacterium. Metab. Eng. 2015, 32, 74–81. https://doi.org/10.1016/j.ymben.2015.09.009.

(3)Crown, S. B.; Long, C. P.; Antoniewicz, M. R. Integrated 13C-Metabolic Flux Analysis of 14 Parallel Labeling Experiments in Escherichia Coli. Metab. Eng. 2015, 28, 151–158. https://doi.org/10.1016/j.ymben.2015.01.001.

(4)Crown, S. B.; Marze, N.; Antoniewicz, M. R. Catabolism of Branched Chain Amino Acids Contributes Significantly to Synthesis of Odd-Chain and Even-Chain Fatty Acids in 3T3-L1 Adipocytes. PLoS ONE 2015, 10 (12), e0145850. https://doi.org/10.1371/journal.pone.0145850.

(5)Doolan, K. M.; Colby, D. W. Conformation-Dependent Epitopes Recognized by Prion Protein Antibodies Probed Using Mutational Scanning and Deep Sequencing. J. Mol. Biol. 2015, 427 (2), 328–340. https://doi.org/10.1016/j.jmb.2014.10.024.

(6)Gebreselassie, N. A.; Antoniewicz, M. R. 13 C-Metabolic Flux Analysis of Co-Cultures: A Novel Approach. Metabolic Engineering 2015, 31, 132–139. https://doi.org/10.1016/j.ymben.2015.07.005.

(7)Haines-Menges, B. L.; Whitaker, W. B.; Lubin, J. B.; Boyd, E. F. Host Sialic Acids: A Delicacy for the Pathogen with Discerning Taste. Microbiol Spectr 2015, 3 (4). https://doi.org/10.1128/microbiolspec.MBP-0005-2014.

(8)Hudson, D. A.; Gannon, S. A.; Thorpe, C. Oxidative Protein Folding: From Thiol–Disulfide Exchange Reactions to the Redox Poise of the Endoplasmic Reticulum. Free Radical Biology and Medicine 2015, 80, 171–182. https://doi.org/10.1016/j.freeradbiomed.2014.07.037.

(9)Hudson, D. A.; Thorpe, C. Mia40 Is a Facile Oxidant of Unfolded Reduced Proteins but Shows Minimal Isomerase Activity. Archives of Biochemistry and Biophysics 2015, 579, 1–7. https://doi.org/10.1016/j.abb.2015.05.005.

(10)Lewis, K. M.; Bharadwaj, U.; Eckols, T. K.; Kolosov, M.; Kasembeli, M. M.; Fridley, C.; Siller, R.; Tweardy, D. J. Small-Molecule Targeting of Signal Transducer and Activator of Transcription (STAT) 3 to Treat Non-Small Cell Lung Cancer. Lung Cancer 2015, 90 (2), 182–190. https://doi.org/10.1016/j.lungcan.2015.09.014.

(11)Lewus, R. A.; Levy, N. E.; Lenhoff, A. M.; Sandler, S. I. A Comparative Study of Monoclonal Antibodies. 1. Phase Behavior and Protein-Protein Interactions. Biotechnol. Prog. 2015, 31 (1), 268–276. https://doi.org/10.1002/btpr.2011.

(12)Lubin, J.-B.; Lewis, W. G.; Gilbert, N. M.; Weimer, C. M.; Almagro-Moreno, S.; Boyd, E. F.; Lewis, A. L. Host-like Carbohydrates Promote Bloodstream Survival of Vibrio Vulnificus in Vivo. Infect. Immun. 2015, 83 (8), 3126–3136. https://doi.org/10.1128/IAI.00345-15.

(13)Melnyk, J. E.; Mohanan, V.; Schaefer, A. K.; Hou, C.-W.; Grimes, C. L. Peptidoglycan Modifications Tune the Stability and Function of the Innate Immune Receptor Nod2. J. Am. Chem. Soc. 2015, 137 (22), 6987–6990. https://doi.org/10.1021/jacs.5b01607.

(14)Monillas, E. S.; Caplan, J. L.; Thévenin, A. F.; Bahnson, B. J. Oligomeric State Regulated Trafficking of Human Platelet-Activating Factor Acetylhydrolase Type-II. Biochim. Biophys. Acta 2015, 1854 (5), 469–475. https://doi.org/10.1016/j.bbapap.2015.02.007.

(15)Ongagna-Yhombi, S. Y.; McDonald, N. D.; Boyd, E. F. Deciphering the Role of Multiple Betaine-Carnitine-Choline Transporters in the Halophile Vibrio Parahaemolyticus. Appl. Environ. Microbiol. 2015, 81 (1), 351–363. https://doi.org/10.1128/AEM.02402-14.

(16)Panish, R.; Selvaraj, R.; Fox, J. M. Rh(II)-Catalyzed Reactions of Diazoesters with Organozinc Reagents. Org. Lett. 2015, 17 (16), 3978–3981. https://doi.org/10.1021/acs.orglett.5b01836.

(17)Ross, N. L.; Munsell, E. V.; Sabanayagam, C.; Sullivan, M. O. Histone-Targeted Polyplexes Avoid Endosomal Escape and Enter the Nucleus During Postmitotic Redistribution of ER Membranes. Mol Ther Nucleic Acids 2015, 4, e226. https://doi.org/10.1038/mtna.2015.2.

2014

(1)Haines-Menges, B.; Whitaker, W. B.; Boyd, E. F. Alternative Sigma Factor RpoE Is Important for Vibrio Parahaemolyticus Cell Envelope Stress Response and Intestinal Colonization. Infect. Immun. 2014, 82 (9), 3667–3677. https://doi.org/10.1128/IAI.01854-14.

(2)He, L.; Xiao, Y.; Gebreselassie, N.; Zhang, F.; Antoniewiez, M. R.; Tang, Y. J.; Peng, L. Central Metabolic Responses to the Overproduction of Fatty Acids in Escherichia Coli Based on 13C-Metabolic Flux Analysis. Biotechnol. Bioeng. 2014, 111 (3), 575–585. https://doi.org/10.1002/bit.25124.

(3)Levy, N. E.; Valente, K. N.; Choe, L. H.; Lee, K. H.; Lenhoff, A. M. Identification and Characterization of Host Cell Protein Product-Associated Impurities in Monoclonal Antibody Bioprocessing. Biotechnol. Bioeng. 2014, 111 (5), 904–912. https://doi.org/10.1002/bit.25158.

(4)Liang, Q.; Dexheimer, T. S.; Zhang, P.; Rosenthal, A. S.; Villamil, M. A.; You, C.; Zhang, Q.; Chen, J.; Ott, C. A.; Sun, H.; et al. A Selective USP1-UAF1 Inhibitor Links Deubiquitination to DNA Damage Responses. Nat. Chem. Biol. 2014, 10 (4), 298–304. https://doi.org/10.1038/nchembio.1455.

(5)Long, C. P.; Antoniewicz, M. R. Metabolic Flux Analysis of Escherichia Coli Knockouts: Lessons from the Keio Collection and Future Outlook. Curr. Opin. Biotechnol. 2014, 28, 127–133. https://doi.org/10.1016/j.copbio.2014.02.006.

(6)Long, C. P.; Antoniewicz, M. R. Quantifying Biomass Composition by Gas Chromatography/Mass Spectrometry. Anal. Chem. 2014, 86 (19), 9423–9427. https://doi.org/10.1021/ac502734e.

(7)Sakowski, E. G.; Munsell, E. V.; Hyatt, M.; Kress, W.; Williamson, S. J.; Nasko, D. J.; Polson, S. W.; Wommack, K. E. Ribonucleotide Reductases Reveal Novel Viral Diversity and Predict Biological and Ecological Features of Unknown Marine Viruses. Proc. Natl. Acad. Sci. U.S.A. 2014, 111 (44), 15786–15791. https://doi.org/10.1073/pnas.1401322111.

(8)Schaefer-Ramadan, S.; Thorpe, C.; Rozovsky, S. Site-Specific Insertion of Selenium into the Redox-Active Disulfide of the Flavoprotein Augmenter of Liver Regeneration. Arch. Biochem. Biophys. 2014, 548, 60–65. https://doi.org/10.1016/j.abb.2014.02.001.

(9)Valente, K. N.; Schaefer, A. K.; Kempton, H. R.; Lenhoff, A. M.; Lee, K. H. Recovery of Chinese Hamster Ovary Host Cell Proteins for Proteomic Analysis. Biotechnol J 2014, 9 (1), 87–99. https://doi.org/10.1002/biot.201300190.

(10)Yang, M.; Lai, S. K.; Yu, T.; Wang, Y.-Y.; Happe, C.; Zhong, W.; Zhang, M.; Anonuevo, A.; Fridley, C.; Hung, A.; et al. Nanoparticle Penetration of Human Cervicovaginal Mucus: The Effect of Polyvinyl Alcohol. J Control Release 2014, 192, 202–208. https://doi.org/10.1016/j.jconrel.2014.07.045.

2013

(1)Crown, S. B.; Antoniewicz, M. R. Parallel Labeling Experiments and Metabolic Flux Analysis: Past, Present and Future Methodologies. Metab. Eng. 2013, 16, 21–32. https://doi.org/10.1016/j.ymben.2012.11.010.

(2)Crown, S. B.; Antoniewicz, M. R. Publishing 13C Metabolic Flux Analysis Studies: A Review and Future Perspectives. Metab. Eng. 2013, 20, 42–48. https://doi.org/10.1016/j.ymben.2013.08.005.

(3)Fisher, L. A.; Smith, N. J.; Fox, J. M. Chiral Cyclopropenyl Ketones: Reactive and Selective Diels-Alder Dienophiles. J. Org. Chem. 2013, 78 (7), 3342–3348. https://doi.org/10.1021/jo302683t.

(4)Panish, R.; Chintala, S. R.; Boruta, D. T.; Fang, Y.; Taylor, M. T.; Fox, J. M. Enantioselective Synthesis of Cyclobutanes via Sequential Rh-Catalyzed Bicyclobutanation/Cu-Catalyzed Homoconjugate Addition. J. Am. Chem. Soc. 2013, 135 (25), 9283–9286. https://doi.org/10.1021/ja403811t.

(5)Rehmann, M. S.; Garibian, A. C.; Kloxin, A. M. Hydrolytically Degradable Thiol-Ene Hydrogels for Protein Release. Macromol Symp 2013, 329 (1), 58–65. https://doi.org/10.1002/masy.201200133.

(6)Rehmann, M. S.; Kloxin, A. M. Tunable and Dynamic Soft Materials for Three-Dimensional Cell Culture. Soft Matter 2013, 9 (29), 6737–6746. https://doi.org/10.1039/C3SM50217A.

(7)Russell, K. C.; Tucker, H. A.; Bunnell, B. A.; Andreeff, M.; Schober, W.; Gaynor, A. S.; Strickler, K. L.; Lin, S.; Lacey, M. R.; O’Connor, K. C. Cell-Surface Expression of Neuron-Glial Antigen 2 (NG2) and Melanoma Cell Adhesion Molecule (CD146) in Heterogeneous Cultures of Marrow-Derived Mesenchymal Stem Cells. Tissue Eng Part A 2013, 19 (19–20), 2253–2266. https://doi.org/10.1089/ten.TEA.2012.0649.

(8)Schaefer, S. A.; Dong, M.; Rubenstein, R. P.; Wilkie, W. A.; Bahnson, B. J.; Thorpe, C.; Rozovsky, S. (77)Se Enrichment of Proteins Expands the Biological NMR Toolbox. J. Mol. Biol. 2013, 425 (2), 222–231. https://doi.org/10.1016/j.jmb.2012.11.011.

(9)Schaefer-Ramadan, S.; Gannon, S. A.; Thorpe, C. Human Augmenter of Liver Regeneration: Probing the Catalytic Mechanism of a Flavin-Dependent Sulfhydryl Oxidase. Biochemistry 2013, 52 (46), 8323–8332. https://doi.org/10.1021/bi401305w.

2012

(1)Crown, S. B.; Ahn, W. S.; Antoniewicz, M. R. Rational Design of 13C-Labeling Experiments for Metabolic Flux Analysis in Mammalian Cells. BMC Syst Biol 2012, 6, 43. https://doi.org/10.1186/1752-0509-6-43.

(2)Crown, S. B.; Antoniewicz, M. R. Selection of Tracers for 13C-Metabolic Flux Analysis Using Elementary Metabolite Units (EMU) Basis Vector Methodology. Metab. Eng. 2012, 14 (2), 150–161. https://doi.org/10.1016/j.ymben.2011.12.005.

(3)Lubin, J.-B.; Kingston, J. J.; Chowdhury, N.; Boyd, E. F. Sialic Acid Catabolism and Transport Gene Clusters Are Lineage Specific in Vibrio Vulnificus. Appl. Environ. Microbiol. 2012, 78 (9), 3407–3415. https://doi.org/10.1128/AEM.07395-11.

2011

(1)Crown, S. B.; Indurthi, D. C.; Ahn, W. S.; Choi, J.; Papoutsakis, E. T.; Antoniewicz, M. R. Resolving the TCA Cycle and Pentose-Phosphate Pathway of Clostridium Acetobutylicum ATCC 824: Isotopomer Analysis, in Vitro Activities and Expression Analysis. Biotechnol J 2011, 6 (3), 300–305. https://doi.org/10.1002/biot.201000282.

(2)Lewis, A. L.; Lubin, J.-B.; Argade, S.; Naidu, N.; Choudhury, B.; Boyd, E. F. Genomic and Metabolic Profiling of Nonulosonic Acids in Vibrionaceae Reveal Biochemical Phenotypes of Allelic Divergence in Vibrio Vulnificus. Appl. Environ. Microbiol. 2011, 77 (16), 5782–5793. https://doi.org/10.1128/AEM.00712-11.

(3)Thévenin, A. F.; Monillas, E. S.; Winget, J. M.; Czymmek, K.; Bahnson, B. J. Trafficking of Platelet-Activating Factor Acetylhydrolase Type II in Response to Oxidative Stress. Biochemistry 2011, 50 (39), 8417–8426. https://doi.org/10.1021/bi200802w.

(4)Thévenin, A. F.; Zony, C. L.; Bahnson, B. J.; Colman, R. F. Activation by Phosphorylation and Purification of Human C-Jun N-Terminal Kinase (JNK) Isoforms in Milligram Amounts. Protein Expr. Purif. 2011, 75 (2), 138–146. https://doi.org/10.1016/j.pep.2010.08.002.

(5)Thévenin, A. F.; Zony, C. L.; Bahnson, B. J.; Colman, R. F. GST Pi Modulates JNK Activity through a Direct Interaction with JNK Substrate, ATF2. Protein Sci. 2011, 20 (5), 834–848. https://doi.org/10.1002/pro.609.

2010

(1)Daithankar, V. N.; Schaefer, S. A.; Dong, M.; Bahnson, B. J.; Thorpe, C. Structure of the Human Sulfhydryl Oxidase Augmenter of Liver Regeneration and Characterization of a Human Mutation Causing an Autosomal Recessive Myopathy. Biochemistry 2010, 49 (31), 6737–6745. https://doi.org/10.1021/bi100912m.

 

 

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