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Engineered hydrogels for mechanobiology

U.Blache, EM.Ford, B.Ha, L.Rijins, O.Chaudhurl, PYW.Dankers, AM. Kloxin,

JG.Snedeker, E.Gentleman

Nature reviews methods primers, (2022) 2:98,

DOI: 10.1038/S43586-022-00179-7, Share it Link:

Dynamic bioinspired coculture model for probing ER+ breast cancer dormancy in the bone marrow niche

L.Pradhan, D.Moore, EM.Ovadia, SL.Swedzinski, T.Cossette, RA.Sikes, KV.Golen,

AM. Kloxin

SCIENCE ADVANCES, 8 Mar 2023, Vol 9Issue 10

Cell Therapy Biomanufacturing: Integrating Biomaterial and Flow-Based Membrane Technologies for Production of Engineered T-Cells

K.BombPJ. LeValleyIR. WoodwardSE. CasselBP. SutherlandA. BhattacharjeeZ. YunJ.SteenE.KurdzoJ.McCoskeyD.BurrisK.LevineC.CarbrelloAM. LenhoffCA. FromenAM. Kloxin

DOI:, Adv. Mater. Technol. 2023, 2201155

Destructive fibrotic teamwork: how both microenvironment stiffness and profibrotic interleukin 13 impair alveolar macrophage phenotype and function
K. Bomb, L. Pradhan, Q. Zhang, BM. Jarai, A. Bhattacharjee, DL. Burris, AM Kloxin, CA Fromen
DOI: 10.1039/D2BM00828A  Biomater. Sci., 2022, 10, 5689-5706


P. LeValley, AL. Parsons, B Sutherland, KL. Kiick, AM Kloxin

Microgels Formed by Spontaneous Click Chemistries Utilizing Microfluidic Flow Focusing for Cargo Release in Response to Endogenous or Exogenous Stimuli

Pharmaceutics 14(5):1062, May 2022


The living interface between synthetic biology and biomaterial design

Allen P. Liu, Eric A. Appel, Paul D. Ashby, Brendon M. Baker, Elisa Franco, Luo Gu, Karmella Haynes, Neel S. Joshi, April M. Kloxin, Paul H. J. Kouwer, Jeetain Mittal, Leonardo Morsut, Vincent Noireaux, Sapun Parekh, Rebecca Schulman, Sindy K. Y. Tang, Megan T. Valentine, Sebastián L. Vega, Wilfried Weber, Nicholas Stephanopoulos & Ovijit Chaudhuri

Nat. Mater. 21, 390–397 (2022).

Rational Design of Hydrogel Networks with Dynamic Mechanical Properties to Mimic Matrix Remodeling
Katherine L. Wiley,Bryan P. Sutherland,Babatunde A. Ogunnaike, April M. Kloxin
Advanced Healthcare Materials,
PA.Taylor, AM Kloxin, A.Jayaraman

“Impact of collagen-like peptide (CLP) heterotrimeric triple helix design on helical thermal stability and hierarchical assembly: a coarse-grained molecular dynamics simulation study” ,

Royal Society of Chemistry, 2022. DOI: 10.1039/D2SM00087C

KA. Wodzanowski, JL. Caplan, AM. Kloxin and CL. Grimes

“Multiscale Invasion Assay for Probing Macrophage Response to Gram-Negative Bacteria”

Front. Chem., 15 February 2022 Sec. Chemical Biology

EM Ford, AM Kloxin, “Rapid production of multifunctional self-assembling peptides for incorporation and visualization within hydrogel biomaterials,” ACS Biomaterials Science & Engineering, 2021. DOI: 10.1021/acsbiomaterials.1c00589

AM Hilderbrand, PA Taylor, F Stanzione, M Larue, C Guo, A Jayaraman, AM Kloxin, “Combining simulations and experiments for the molecular engineering of multifunctional collagen mimetic peptide-based materials,” Soft Matter, 17, 1985-1998, 2021. DOI:10.1039/d0sm01562h

BM Jarai, Z Stillman, K Bomb, AM Kloxin, CA Fromen, “Biomaterials-Based Opportunities to Engineer the Pulmonary Host Immune Response in COVID-19,” ACS Biomaterials Science & Engineering, 7, 1742-1764, 2021. DOI:10.1021/acsbiomaterials.0c01287

T Sulerud, AB Sami, G Li, AM Kloxin, J Oakey, J Gatlin, “Microtubule-dependent pushing forces contribute to long-distance aster movement and centration in Xenopus laevis egg extracts,” Molecular Biology of the Cell, 31, 2791–2802, 2020. DOI:10.1091/mbc.e20-01-0088

RC Locke, EM Ford, KG Silbernagel, AM Kloxin, ML Killian, “Success criteria and preclinical testing of multifunctional hydrogels for tendon regeneration,” Tissue Engineering Part C: Methods, 26, 10, 506-518, 2020. DOI: 10.1089/ten.tec.2020.0199

PJ LeValley, BO Sutherland, J Jaje, S Gibbs, M Jones, R Gala, CJ Kloxin, KL Kiick, AM Kloxin, “On-demand and tunable dual wavelength release of antibodies using light-responsive hydrogels,” ACS Applied Bio Materials, 3, 10, 6944-6958, 2020. DOI: 10.1021/acsabm.0c00823

BP Sutherland, PJ LeValley, DJ Bischoff, AM Kloxin, CJ Kloxin, “Sequence-defined vinyl sulfonamide click nucleic acids (VS-CNAs) and their assembly into dynamically responsive materials,” Chemical Communications, 56, 11263-11266, 2020. DOI: 10.1039/D0CC04235H

EM Ovadia, L Pradhan, LA Sawicki, JE Cowart, RE Huber, SW Polson, C Chen, KL van Golen, KE Ross, CH Wu, AM Kloxin, “Understanding ER+ Breast Cancer Dormancy using Bioinspired Synthetic Matrices for Long-term 3D Culture and Insights into Late Recurrence,” Advanced Biosystems, 4, 9, 2000119, 2020. DOI: 10.1002/adbi.202000119

SJ Ma, EM Ford, LA Sawicki, BP Sutherland, NI Halaszynski, BJ Carberry, NJ Wagner, AM Kloxin, CJ Kloxin, “Surface Chemical Functionalization of Wrinkled Thiol–Ene Elastomers for Promoting Cellular Alignment,” ACS Applied Bio Materials, 3, 6, 3731-3740, 2020. DOI: 10.1021/acsabm.0c00346

KA Wodzanowski, SE Cassel, CL Grimes, AM Kloxin, “Tools for probing host-bacteria interactions in the gut microenvironment: from molecular to cellular levels,” Bioorganic & Medicinal Chemistry Letters, 30, 127116, 2020. DOI: 10.1016/j.bmcl.2020.127116

PJ LeValley, R Neelarapu, BP Sutherland, S Dasgupta, CJ Kloxin, AM Kloxin, “Photolabile Linkers: Exploiting labile bond chemistry to control mode and rate of hydrogel degradation and protein release,” Journal of the American Chemical Society, 142, 10, 4671-4679, 2020. DOI: 10.1021/jacs.9b11564

AM Hilderbrand, EM Ford, C Guo, JD Sloppy, AM Kloxin, “Hierarchically structured hydrogels utilizing multifunctional assembling peptides for 3D cell culture,” Biomaterials Science, 8, 1256-1269, 2020. DOI: 10.1039/C9BM01894H

Alina I Marusina, Alexander A Merleev, Jesus I Luna, Laura Olney, Nathan E Haigh, Daniel Yoon, Chen Guo, Elisa M Ovadia, Michiko Shimoda, Guillaume Luxardi, Sucharita Boddu, Nelvish N Lal, Yoshikazu Takada, Kit S Lam, Ruiwu Liu, R Rivkah Isseroff, Stephanie Le, Jan A Nolta, April M Kloxin, Emanual Maverakis, “Tunable hydrogels for mesenchymal stem cell delivery: Integrin‐induced transcriptome alterations and hydrogel optimization for human wound healing,” Stem Cells, 38, 231-245, 2020. DOI: 10.1002/stem.3105

ME Smithmyer, SE Cassel, AM Kloxin, “Bridging 2D and 3D culture: Probing impact of extracellular environment on fibroblast activation in layered hydrogels,” AIChE Journal, 65, e16837, 2019. DOI: 10.1002/aic.16837

AM Kloxin, “Addressing the leaky pipeline through mentoring and support: a personal perspective,” Nature Reviews Materials, 4, 287-289, 2019. DOI: 10.1038/s41578-019-0109-0

Jitender Bisht, Paige LeValley, Benjamin Noren, Ralph McBride, Prathamesh Kharkar, April Kloxin, Jesse Gatlin, John Oakey, “Light-inducible activation of cell cycle progression in Xenopus egg extracts under microfluidic confinement,” Lab on a Chip, 19, 3499-3511, 2019. DOI: 10.1039/C9LC00569B

KL Wiley, EM Ovadia, CJ Calo, RE Huber, AM Kloxin, “Rate-based approach for controlling the mechanical properties of ‘thiol–ene’ hydrogels formed with visible light,” Polymer Chemistry, 10, 4428-4440, 2019. DOI: 10.1039/C9PY00447E

LA Sawicki, EM Ovadia, L Pradhan, JE Cowart, KE Ross, CH Wu, AM Kloxin, “Tunable synthetic extracellular matrices to investigate breast cancer response to biophysical and biochemical cues,” APL Bioengineering, 3, 016101, 2019. DOI: 10.1063/1.5064596

PJ LeValley, AM Kloxin, “Chemical Approaches to Dynamically Modulate the Properties of Synthetic Matrices,”ACS Macro Letters, 8, 7-16, 2019. DOI: 10.1021/acsmacrolett.8b00808

PJ LeValley, MW Tibbitt, B Noren, PM Kharkar, AM Kloxin, KS Anseth, M Toner, J Oakey “Immunofunctional photodegradable poly(ethylene glycol) hydrogel surfaces for the capture and release of rare cells,” Colloids and Surfaces B: Biointerfaces, 174, 483-492, 2019. DOI: 10.1016/j.colsurfb.2018.11.049

H Wu, PJ LeValley, T, Luo, AM Kloxin, KL Kiick, “Manipulation of Glutathione-Mediated Degradation of Thiol-Maleimide Conjugates,” Bioconjugate Chemistry, 29, 3595-3605, 2018. DOI: 10.1021/acs.bioconjchem.8b00546

ME Smithmyer, CC Deng, SE Cassel, PJ LeValley, BS Sumerlin, AM Kloxin, “Self-healing boronic acid-based hydrogels for 3D co-cultures,” ACS Macro Letters, 7, 1105-1110, 2018. DOI: 10.1021/acsmacrolett.8b00462

ME Smithmyer, JB Spohn, AM Kloxin, “Probing fibroblast activation in response to extracellular matrix cues with well-defined culture platform,” ACS Biomaterials Science & Engineering, 4, 3304-3316, 2018. DOI: 10.1021/acsbiomaterials.8b00491

PJ LeValley, B Noren, PM Kharkar, AM Kloxin, JC Gatlin, J Oakey, “Fabrication of Complex Functional Biomaterial Microstructures by In situ Photopolymerization and Photodegradation,” ACS Biomaterials Science & Engineering, 4, 3078-3087, 2018. DOI: 10.1021/acsbiomaterials.8b00350

PJ LeValley, EM Ovadia, CA Bresette, LA Sawicki, E Maverakis, S Bai, AM Kloxin, “Design of functionalized cyclic peptides through orthogonal click reactions for cell culture and targeting applications,” Chemical Communications, 54, 6923-6926, 2018. DOI: 10.1039/C8CC03218A.

LJ Macdougall, KL Wiley, AM Kloxin, AP Dove, “Design of Synthetic Extracellular Matrices for Probing Breast Cancer Cell Growth Using Robust Cyctocompatible Nucleophilic Thiol-yne Addition Chemistry,” Biomaterials, 178, 435-447, 2018. DOI: 10.1016/j.biomaterials.2018.04.046.

EM Ovadia, DW Colby, AM Kloxin, “Designing photopolymerized synthetic matrices for three-dimensional culture and differentiation of induced pluripotent stem cells,” Biomaterials Science, 6, 1358-1370, 2018. DOI: 10.1039/c8bm00099a

NJ Smith, K Rohlfing, LA Sawicki, PM Kharkar, SJ Boyd, AM Kloxin, JM Fox, “Fast, Irreversible Modification of Cysteines through Strain Releasing Conjugate Additions of Cyclopropenyl Ketones,” Organic & Biomolecular Chemistry, 16, 2164-2169, 2018. DOI: 10.1039/C8OB00166A

slide1LA Sawicki, LH Choe, KL Wiley, KH Lee, AM Kloxin, “Techniques for the isolation and identification of proteins secreted by cells cultured within synthetic extracellular matrices,” ACS Biomaterials Science and Engineering, 4, 836-845, 2018, 2018.       DOI: 10.1021/acsbiomaterials.7b00647

slide1 PM Kharkar, RA Scott, LP Olney, PF LeValley, E Maverakis, KL Kiick, AM Kloxin, “Controlling the release of small, bioactive proteins via dual mechanisms with therapeutic potential,” Advanced Healthcare Materials, 6, 1700713, 2017   DOI: 10.1002/adhm.201700713

slide1 MS Rehmann, KM Skeens, PM Kharkar, E Maverakis, KH Lee, AM Kloxin, “Tuning and predicting mesh size and protein release from step growth hydrogels,” Biomacromolecules, 18, 3131-3142, 2017    DOI: 10.1021/acs.biomac.7b00781

slide1 C Guo*, H Kim*, EM Ovadia*, CM Mourafetis, M Yang, W Chen, AM Kloxin, “Bio-orthogonal conjugation and enzymatically triggered release of proteins within multi-layered hydrogels,” Acta Biomaterialia, 2017 DOI: 10.1016/j.actbio.2017.04.002

slide1AM Hilderbrand*, EM Ovadia*, MS Rehmann, PM Kharkar, C Guo, AM Kloxin, “Biomaterials for 4D Stem Cell Culture,” Current Opinion in Solid State and Materials Science, 2016 DOI: 10.1016/j.cossms.2016.03.002

Sawicki 2016

LA Sawicki, AM Kloxin, “Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications,” Journal of Visualized Experiments, 2016, DOI: 10.3791/54462

MS Rehmann, JI Luna, E Maverakis, Rehmann 2016AM Kloxin, “Tuning microenvironment modulus and biochemical composition promotes human mesenchymal stem cell tenogenic differentiation,” Journal of Biomedical Materials Research Part A, 2016 DOI: 10.1002/jbm.a.35650

Kharkar 2016

PM Kharkar, MS Rehmann, KM Skeens, E Maverakis, AM Kloxin, “Thiol–ene Click Hydrogels for Therapeutic Delivery,” ACS Biomaterials Science and Engineering, 2016 DOI: 10.1021/acsbiomaterials.5b00420

Maverakis 2016

KA Tran, MY Cheng, A Mitra, H Ogawa, VY Shi, LP Olney, AM Kloxin, and E Maverakis, “MEK inhibitors and their potential in the treatment of advanced melanoma: the advantages of combination therapy,” Drug Design, Development and Therapy, 2016 DOI: 10.2147/DDDT.S93545

Sawicki 2015

LA Sawicki, AM Kloxin, “Biomaterials: Controlling Properties Over Time to Mimic the Dynamic Extracellular Matrix,” Royal Society of Chemistry, 2015 ISBN: 978-1-84973-833-0

Kharkar 2015

PM Kharkar, KL Kiick, AM Kloxin, “Design of thiol- and light-sensitive degradable hydrogels using Michael-type addition reactions,” Polymer Chemistry, 2015 DOI: 10.1039/C5PY00750J

Maverakis 2015

E Maverakis, LA Cornelius, GM Bowen, T Phan, FB Patel, S Fitzmaurice, Y He, B Burrall, C Duong, AM Kloxin, H Sultani, R Wilken, SR Martinez, F Patel, “Metastatic Melanoma – A Review of Current and Future Treatment Options,” Acta Dermato-Venereologica, 2015 DOI: 10.2340/00015555-2035

LA Sawicki, AM Kloxin, “Design of thiol–ene photoclick hydrogels using facile techniques for cell culture applications,” Biomaterials Science, 2014 DOI: 10.1039/C4BM00187G

Kharkar J Mater Chem B 2014

PM Kharkar, AM Kloxin, KL Kiick, “Dually degradable click hydrogels for controlled degradation and protein release,” Journal of Materials Chemistry B, 2014 DOI: 10.1039/C4TB00496E

Smithmyer Biomaterials Science 2014

ME Smithmyer, LA Sawicki, AM Kloxin, “Hydrogel scaffolds as in vitro models to study fibroblast activation in wound healing and disease,” Biomaterials Science, 2014. DOI: 10.1039/C3BM60319A

Rehmann Macromolecular Symposia 2013

MS Rehmann, AC Garibian, AM Kloxin, “Hydrolytically Degradable Thiol–ene Hydrogels for Protein Release,” Macromolecular Symposia, 329, 58–65, 2013. DOI: 10.1002/masy.201200133

Kharkar Chem Soc Rev 2013

PM Kharkar, KL Kiick, AM Kloxin, “Designing degradable hydrogels for orthogonal control of cell microenvironments,” Chemical Society Reviews, 42, 7335-7372, 2013. DOI: 10.1039/C3CS60040H

Rehmann Soft Matter 2013

MS Rehmann, AM Kloxin, “Tunable and dynamic soft materials for three-dimensional cell culture,” Soft Matter, 9, 6737-6746, 2013.  DOI: 10.1039/C3SM50217A

Tibbitt Macromolecules 2013

MW Tibbitt, AM Kloxin, LA Sawicki, KS Anseth, “Mechanical Properties and Degradation of Chain and Step Polymerized Photodegradable Hydrogels,” Macromolecules, 46, 2785-2792, 2013. DOI:10.1021/ma302522x

McKinnon Biomaterials Science 2013

DD McKinnon, AM Kloxin, KS Anseth, “Synthetic hydrogel platform for three-dimensional culture of embryonic stem cell-derived motor neurons,” Biomaterials Science, 1, 460-469, 2013.  DOI: 10.1039/C3BM00166K

Tibbitt J Poly Sci 2013

MW Tibbitt, AM Kloxin, KS Anseth, “Modeling Controlled Photodegradation in Optically Thick Hydrogels,” Journal of Polymer Science Part A: Polymer Chemistry, 51, 1899-1911, 2013. DOI: 10.1002/pola.26574

Kloxin Int Bio 2012

AM Kloxin, KJR Lewis, CA DeForest, MW Tibbitt, V Balasubramaniam, and KS Anseth, “Responsive culture platform to examine the influence of microenvironmental geometry on cell function in 3D,” Integrative Biology, 4, 1540-1549, 2012. DOI:10.1039/C2IB20212C

Wang PLoS One 2012

H Wang, SM Haeger, AM Kloxin, LA Leinwand, KS Anseth, “Redirecting Valvular Myofibroblasts into Dormant Fibroblasts through Light-mediated Reduction in Substrate Modulus,” PLoS One, 7, e39969, 2012. DOI:10.1371/journal.pone.0039969

Tibbitt J Biomed Maters Res 2012

MW Tibbitt, BW Han, AM Kloxin, KS Anseth, “Synthesis and application of photodegradable microspheres for spatiotemporal control of protein delivery,” Journal of Biomedical Materials Research Part A, 100, 1647-1654, 2012. DOI: 10.1002/jbm.a.34107

Kloxin Nat Protoc 2010

AM Kloxin, MW Tibbitt, and KS Anseth, “Synthesis of photodegradable hydrogels as dynamically tunable cell culture platforms,” Nature Protocols, 5, 1867-1887, 2010. DOI: 10.1038/nprot.2010.139

Tibbitt Soft Matter 2010

MW Tibbitt, AM Kloxin, KU Dyamenahalli, and KS Anseth, “Controlled two-photon photoerosion of PEG hydrogels to study and manipulate subcellular interactions on soft materials,” Soft Matter, 6, 5100-5108, 2010. DOI: 10.1039/C0SM00174K

Kloxin Adv Mater 2010 1

AM Kloxin, CJ Kloxin, CN Bowman, and KS Anseth, “Mechanical properties of cellularly responsive hydrogels and their experimental determination,” Advanced Materials, 22, 3484-3494, 2010. DOI: 10.1002/adma.200904179

Kloxin Adv Mater 2010 2

AM Kloxin, MW Tibbitt, AM Kasko, JA Fairbairn, and KS Anseth, “Tunable hydrogels through controlled photodegradation for external manipulation of the cell microenvironment,” Advanced Materials, 22, 61-66, 2010. DOI: 10.1002/adma.200900917

Kloxin Biomaters 2009

AM Kloxin, JA Benton, and KS Anseth, “Photodegradable hydrogels as dynamic substrates for probing the influence of elasticity on cell phenotypes,” Biomaterials, 31, 1-8, 2010. DOI:10.1016/j.biomaterials.2009.09.025

Kloxin Science 2009

AM Kloxin, AM Kasko, CN Salinas, and KS Anseth, “Photolabile hydrogels for dynamic tuning of physical and chemical properties,” Science, 324, 59-63, 2009. DOI: 10.1126/science.1169494

Khire 2008

VS Khire, AM Kloxin, CC Clouch, KS Anseth, and CN Bowman, “Synthesis, Characterization and Cleavage of linear polymers attached to Silica Nanoparticles formed using Thiol-acrylate Conjugate Addition Reactions,” Journal of Polymer Science A: Polymer Chemistry, 46, 6896-6906, 2008. DOI: 10.1002/pola.22999

AM Kloxin and KS Anseth, “Protein gels on the move,” Nature, 454, 705-706, 2008. [News & Views] DOI: 10.1038/454705a

CR Nuttelman, AM Kloxin, and KS Anseth, “Temporal changes in PEG hydrogel structure influence human mesenchymal stem cell proliferation and matrix mineralization,” Advances in Experimental Medicine and Biology: Tissue Engineering, 585, 135-149, 2006. DOI: 10.1007/978-0-387-34133-0_10