slide1 LA Sawicki, LH Choe, KL Wiley, KH Lee, AM Kloxin, “Techniques for the isolation and identification of proteins secreted by cells cultured within synthetic extracellular matrices,” ACS Biomaterials Science and Engineering, In Press, 2018          DOI: 10.1021/acsbiomaterials.7b00647




slide1 PM Kharkar, RA Scott, LP Olney, PF Levalley, E Maverakis, KL Kiick, AM Kloxin, “Controlling the release of small, bioactive proteins via dual mechanisms with therapeutic potential,” Advanced Healthcare Materials, 6, 1700713, 2017   DOI: 10.1002/adhm.201700713



slide1 MS Rehmann, KM Skeens, PM Kharkar, E Maverakis, KH Lee, AM Kloxin, “Tuning and predicting mesh size and protein release from step growth hydrogels,” Biomacromolecules, 18, 3131-3142, 2017    DOI: 10.1021/acs.biomac.7b00781


slide1 C Guo*, H Kim*, EM Ovadia*, CM Mourafetis, M Yang, W Chen, AM Kloxin, “Bio-orthogonal conjugation and enzymatically triggered release of proteins within multi-layered hydrogels,” Acta Biomaterialia, 2017 DOI: 10.1016/j.actbio.2017.04.002



slide1AM Hilderbrand*, EM Ovadia*, MS Rehmann, PM Kharkar, C Guo, AM Kloxin, “Biomaterials for 4D Stem Cell Culture,” Current Opinion in Solid State and Materials Science, 2016 DOI: 10.1016/j.cossms.2016.03.002





Sawicki 2016LA Sawicki, AM Kloxin, “Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications,” Journal of Visualized Experiments, 2016, DOI: 10.3791/54462






MS Rehmann, JI Luna, E Maverakis, Rehmann 2016AM Kloxin, “Tuning microenvironment modulus and biochemical composition promotes human mesenchymal stem cell tenogenic differentiation,” Journal of Biomedical Materials Research Part A, 2016 DOI: 10.1002/jbm.a.35650



Kharkar 2016

PM Kharkar, MS Rehmann, KM Skeens, E Maverakis, AM Kloxin, “Thiol–ene Click Hydrogels for Therapeutic Delivery,” ACS Biomaterials Science and Engineering, 2016 DOI: 10.1021/acsbiomaterials.5b00420



Maverakis 2016



KA Tran, MY Cheng, A Mitra, H Ogawa, VY Shi, LP Olney, AM Kloxin, and E Maverakis, “MEK inhibitors and their potential in the treatment of advanced melanoma: the advantages of combination therapy,” Drug Design, Development and Therapy, 2016 DOI: 10.2147/DDDT.S93545






Sawicki 2015



LA Sawicki, AM Kloxin, “Biomaterials: Controlling Properties Over Time to Mimic the Dynamic Extracellular Matrix,” Royal Society of Chemistry, 2015 ISBN: 978-1-84973-833-0





Kharkar 2015


PM Kharkar, KL Kiick, AM Kloxin, “Design of thiol- and light-sensitive degradable hydrogels using Michael-type addition reactions,” Polymer Chemistry, 2015 DOI: 10.1039/C5PY00750J





Maverakis 2015


E Maverakis, LA Cornelius, GM Bowen, T Phan, FB Patel, S Fitzmaurice, Y He, B Burrall, C Duong, AM Kloxin, H Sultani, R Wilken, SR Martinez, F Patel, “Metastatic Melanoma – A Review of Current and Future Treatment Options,” Acta Dermato-Venereologica, 2015 DOI: 10.2340/00015555-2035







LA Sawicki, AM Kloxin, “Design of thiol–ene photoclick hydrogels using facile techniques for cell culture applications,” Biomaterials Science, 2014 DOI: 10.1039/C4BM00187G






Kharkar J Mater Chem B 2014

PM Kharkar, AM Kloxin, KL Kiick, “Dually degradable click hydrogels for controlled degradation and protein release,” Journal of Materials Chemistry B, 2014 DOI: 10.1039/C4TB00496E



Smithmyer Biomaterials Science 2014

ME Smithmyer, LA Sawicki, AM Kloxin, “Hydrogel scaffolds as in vitro models to study fibroblast activation in wound healing and disease,” Biomaterials Science, 2014. DOI: 10.1039/C3BM60319A





Rehmann Macromolecular Symposia 2013

MS Rehmann, AC Garibian, AM Kloxin, “Hydrolytically Degradable Thiol–ene Hydrogels for Protein Release,” Macromolecular Symposia, 329, 58–65, 2013. DOI: 10.1002/masy.201200133





Kharkar Chem Soc Rev 2013


PM Kharkar, KL Kiick, AM Kloxin, “Designing degradable hydrogels for orthogonal control of cell microenvironments,” Chemical Society Reviews, 42, 7335-7372, 2013. DOI: 10.1039/C3CS60040H


Rehmann Soft Matter 2013


MS Rehmann, AM Kloxin, “Tunable and dynamic soft materials for three-dimensional cell culture,” Soft Matter, 9, 6737-6746, 2013.  DOI: 10.1039/C3SM50217A





Tibbitt Macromolecules 2013


MW Tibbitt, AM Kloxin, LA Sawicki, KS Anseth, “Mechanical Properties and Degradation of Chain and Step Polymerized Photodegradable Hydrogels,” Macromolecules, 46, 2785-2792, 2013. DOI:10.1021/ma302522x


McKinnon Biomaterials Science 2013


DD McKinnon, AM Kloxin, KS Anseth, “Synthetic hydrogel platform for three-dimensional culture of embryonic stem cell-derived motor neurons,” Biomaterials Science, 1, 460-469, 2013.  DOI: 10.1039/C3BM00166K




Tibbitt J Poly Sci 2013


MW Tibbitt, AM Kloxin, KS Anseth, “Modeling Controlled Photodegradation in Optically Thick Hydrogels,” Journal of Polymer Science Part A: Polymer Chemistry, 51, 1899-1911, 2013. DOI: 10.1002/pola.26574



Kloxin Int Bio 2012


AM Kloxin, KJR Lewis, CA DeForest, MW Tibbitt, V Balasubramaniam, and KS Anseth, “Responsive culture platform to examine the influence of microenvironmental geometry on cell function in 3D,” Integrative Biology, 4, 1540-1549, 2012. DOI:10.1039/C2IB20212C



Wang PLoS One 2012


H Wang, SM Haeger, AM Kloxin, LA Leinwand, KS Anseth, “Redirecting Valvular Myofibroblasts into Dormant Fibroblasts through Light-mediated Reduction in Substrate Modulus,” PLoS One, 7, e39969, 2012. DOI:10.1371/journal.pone.0039969


Tibbitt J Biomed Maters Res 2012


MW Tibbitt, BW Han, AM Kloxin, KS Anseth, “Synthesis and application of photodegradable microspheres for spatiotemporal control of protein delivery,” Journal of Biomedical Materials Research Part A, 100, 1647-1654, 2012. DOI: 10.1002/jbm.a.34107





Kloxin Nat Protoc 2010


AM Kloxin, MW Tibbitt, and KS Anseth, “Synthesis of photodegradable hydrogels as dynamically tunable cell culture platforms,” Nature Protocols, 5, 1867-1887, 2010. DOI: 10.1038/nprot.2010.139





Tibbitt Soft Matter 2010


MW Tibbitt, AM Kloxin, KU Dyamenahalli, and KS Anseth, “Controlled two-photon photoerosion of PEG hydrogels to study and manipulate subcellular interactions on soft materials,” Soft Matter, 6, 5100-5108, 2010. DOI: 10.1039/C0SM00174K



Kloxin Adv Mater 2010 1


AM Kloxin, CJ Kloxin, CN Bowman, and KS Anseth, “Mechanical properties of cellularly responsive hydrogels and their experimental determination,” Advanced Materials, 22, 3484-3494, 2010. DOI: 10.1002/adma.200904179





Kloxin Adv Mater 2010 2


AM Kloxin, MW Tibbitt, AM Kasko, JA Fairbairn, and KS Anseth, “Tunable hydrogels through controlled photodegradation for external manipulation of the cell microenvironment,” Advanced Materials, 22, 61-66, 2010. DOI: 10.1002/adma.200900917




Kloxin Biomaters 2009


AM Kloxin, JA Benton, and KS Anseth, “Photodegradable hydrogels as dynamic substrates for probing the influence of elasticity on cell phenotypes,” Biomaterials, 31, 1-8, 2010. DOI:10.1016/j.biomaterials.2009.09.025





Kloxin Science 2009


AM Kloxin, AM Kasko, CN Salinas, and KS Anseth, “Photolabile hydrogels for dynamic tuning of physical and chemical properties,” Science, 324, 59-63, 2009. DOI: 10.1126/science.1169494


Khire 2008


VS Khire, AM Kloxin, CC Clouch, KS Anseth, and CN Bowman, “Synthesis, Characterization and Cleavage of linear polymers attached to Silica Nanoparticles formed using Thiol-acrylate Conjugate Addition Reactions,” Journal of Polymer Science A: Polymer Chemistry, 46, 6896-6906, 2008. DOI: 10.1002/pola.22999



AM Kloxin and KS Anseth, “Protein gels on the move,” Nature, 454, 705-706, 2008. [News & Views] DOI: 10.1038/454705a

CR Nuttelman, AM Kloxin, and KS Anseth, “Temporal changes in PEG hydrogel structure influence human mesenchymal stem cell proliferation and matrix mineralization,” Advances in Experimental Medicine and Biology: Tissue Engineering, 585, 135-149, 2006. DOI: 10.1007/978-0-387-34133-0_10